Panoramic Parvovirus Strain Discovery Data Chain
| ParvoDB ID | PV011353 | GenBank Accession | EF686010 |
| Subfamily | Parvovirinae | Genus | Bocaparvovirus |
| Species | Bocaparvovirus primate1 | Sequence Length | 293 |
| Definition | Human bocavirus isolate NZ2003_4 noncapsid protein-1 (NP-1) gene, partial cds. | Submission Date | 2007-06-18 |
| Strain Name | NZ2003_4 | Sampling Date | 2003 |
| Sampling Country | New Zealand | Location | New Zealand |
| Submitted By | Kirman,J.R., Redshaw,N., Rich,F.J., Wood,C. and Grimwood,K. | Submitting Institution | Infectious Diseases, Malaghan Institute, Central Services Building, Entrance 7 Victoria University, Kelburn Parade, Wellington 6005, New Zealand |
| Original Host | Homo sapiens | Sample Type | nasopharyngeal aspirates |
| Standardized Host Name | Homo sapiens | Host Tax Rank | species |
| Standardized Host Class | Mammalia | Standardized Host Order | Primates |
| Environmental Origin | - N/A - | Reagent Origin | - N/A - |
| Number | Reagent/Consumables | Purpose | PMID/Url | Reference |
|---|---|---|---|---|
| 1 | High Pure Viral Nucleic Acid kit (Roche Diagnostics, Auckland, NZ) | Nucleic acid extraction from thawed nasopharyngeal aspirates. | 18217577 | View Related Publication |
| 2 | Primers 188F (5′-GAGCTCTGTAAGTACTATTAC-3′) and 542R (5′-CTCTGTGTTGACTGAATACAG-3′) | Amplification of the HBoV nonstructural protein (NP-1) gene by PCR. | ||
| 3 | Expand High Fidelity DNA Polymerase (Roche Diagnostics, Basel, Switzerland) | Used for PCR amplification. | ||
| 4 | ABI3730 Genetic Analyzer | Sequencing of PCR products. | ||
| 5 | BigDye Terminator version 3.1 Ready Reaction Cycle Sequencing kit (Applied Biosystems, Foster City, CA, USA) | Used for sequencing reactions. |