Panoramic Parvovirus Strain Discovery Data Chain
| ParvoDB ID | PV011107 | GenBank Accession | JN990583 |
| Subfamily | Parvovirinae | Genus | Bocaparvovirus |
| Species | Bocaparvovirus primate1 | Sequence Length | 519 |
| Definition | Human bocavirus isolate 9580 VP1 gene, partial cds. | Submission Date | 2011-11-03 |
| Strain Name | 9580 | Sampling Date | 2010-01 |
| Sampling Country | Japan | Location | Japan |
| Submitted By | Khamrin,P., Thongprachum,A., Shimizu,H., Okitsu,S., Hayakawa,S., Maneekarn,N. and Ushijima,H. | Submitting Institution | Department of Microbiology, Faculty of Medicine, Chiang Mai University, 110 Intawarorose, Sriphoom, Muang, Chiang Mai 50200, Thailand |
| Original Host | Homo sapiens | Sample Type | fecal specimens |
| Standardized Host Name | Homo sapiens | Host Tax Rank | species |
| Standardized Host Class | Mammalia | Standardized Host Order | Primates |
| Environmental Origin | - N/A - | Reagent Origin | - N/A - |
| Number | Reagent/Consumables | Purpose | PMID/Url | Reference |
|---|---|---|---|---|
| 1 | QIAamp viral RNA Mini Kit (Qiagen, Hilden, Germany) | Extraction of viral genomes from 10% fecal suspension supernatant | 22499013 | View Related Publication |
| 2 | PCR primers | Detection of bocavirus in stool specimens by polymerase chain reaction (PCR) | ||
| 3 | AK-VP-F1 (50-CGCCGTGGCTCCTGCTCT-30) | Forward primer used in combination for the first PCR | ||
| 4 | AK-VP-R1 (50-TGTTCGCCATCACAAAAGATGTG-30) | Reverse primer used in combination for the first PCR | ||
| 5 | AK-VP-F2 (50-GGCTCCTGCTCTAGGAAATAAAGAG-30) | Forward primer used in nested-PCR | ||
| 6 | AK-VP-R2 (50-CCTGCTGTTAGGTCGTTGTTGTATGT-30) | Reverse primer used in nested-PCR targeting the partial VP1 region of HBoV | ||
| 7 | Wizard SV Gel and PCR Clean-Up System (Promega, Madison, WI) | Purification of PCR amplicons | ||
| 8 | BigDye Terminator Cycle Sequencing Kit (Applied Biosystems, Foster City, CA) | Sequencing of purified PCR amplicons |