|
1
|
QIAamp DNA Mini kit (Qiagen, Hiden, Germany, Cat No. 51306)
|
DNA extraction from nasopharyngeal swab specimens
|
28716110
|
View Related Publication
|
|
2
|
Respiratory Multi Well System r-gene (BioMerieux, Lyon, France, Cat No. 1092480)
|
Detection of human Adenovirus and HBoV using duplex real time PCR
|
|
3
|
MgCl2 at 50 mM
|
Component of the PCR mixture to amplify a fragment of the VP1/2 capsid gene
|
|
4
|
dNTPs at 10 mM
|
Component of the PCR mixture to amplify a fragment of the VP1/2 capsid gene
|
|
5
|
VP-A sense primer (5′-GCACTTCTGTATCAGATGCCTT-3′) at 10 μM
|
Primer used in the first semi-nested PCR to amplify VP1/2 capsid gene
|
|
6
|
VP-B reverse primer (5′-CGTGGTATGTAGGCGTGTAG-3′) at 10 μM
|
Primer used in the first semi-nested PCR to amplify VP1/2 capsid gene
|
|
7
|
10× PCR buffer
|
Component of the PCR mixture to amplify a fragment of the VP1/2 capsid gene
|
|
8
|
W1 solution at 1%
|
Component of the PCR mixture to amplify a fragment of the VP1/2 capsid gene
|
|
9
|
Taq DNA Polymerase (Invitrogen, USA, Cat No. 10342053)
|
Enzyme used in the PCR to amplify a fragment of the VP1/2 capsid gene
|
|
10
|
VP-C primer (5′-CTTAGAACTGGTGAGAGCACTG-3′)
|
Primer used in the second semi-nested PCR to improve the sensitivity of the VP1/2 capsid gene amplification
|
|
11
|
Agarose gel
|
Electrophoresis medium used to observe the VP1/2 capsid PCR product
|
|
12
|
Big Dye Terminator cycle sequencing (Applied Biosystems, Foster City, USA, Cat No. 4337454)
|
Purification and sequencing of VP1/2 capsid PCR products
|
|
13
|
Applied Biosystems AB3100 genetic analyzer
|
Electrophoresis and data collection of the sequenced VP1/2 capsid PCR products
|
