Panoramic Parvovirus Strain Discovery Data Chain
| ParvoDB ID | PV010944 | GenBank Accession | OQ731791 |
| Subfamily | Parvovirinae | Genus | Bocaparvovirus |
| Species | Bocaparvovirus primate2 | Sequence Length | 389 |
| Definition | Human bocavirus 3 isolate Iran/MHKN/HBoV-3/24 capsid protein (VP1) gene, partial cds. | Submission Date | 2023-04-01 |
| Strain Name | Iran/MHKN/HBoV-3/24 | Sampling Date | from 2021 to 2022 |
| Sampling Country | Iran | Location | a pediatric hospital in Tehran, Iran, Iran |
| Submitted By | Karbalaie Niya,M.H., Kachooei,A., Khales,P., Rahmani Fard,S., Hamidzade,M. and Tavakoli,A. | Submitting Institution | Gastrointestinal and Liver Diseases Research Center, Iran University of Medical Sciences, Firoozgar Hospital, Tehran, Tehran 1449614535, Iran |
| Original Host | Homo sapiens | Sample Type | stool samples |
| Standardized Host Name | Homo sapiens | Host Tax Rank | species |
| Standardized Host Class | Mammalia | Standardized Host Order | Primates |
| Environmental Origin | - N/A - | Reagent Origin | - N/A - |
| Number | Reagent/Consumables | Purpose | PMID/Url | Reference |
|---|---|---|---|---|
| 1 | Viga Sars-CoV-2 Molecular Diagnostic kit (ROJE Technologies) | To examine the presence of SARS-CoV-2 infection by real-time reverse transcription PCR assay. | 37779667 | View Related Publication |
| 2 | Phosphate-buffered saline (pH 7.2) | To prepare stool suspensions as 10% (wt/vol) and clear by centrifugation at 2500g for 5 min. | ||
| 3 | HiPurA Multi-Sample DNA Purification kit (HiMedia) | For DNA extraction from stool suspensions as per the manufacturer's instructions. | ||
| 4 | 2x red PCR Master Mix (Ampliqon) | To prepare the PCR reaction mix for amplifying the VP1 gene. | ||
| 5 | DNase and RNase-free water | To dilute the PCR reaction mix appropriately. | ||
| 6 | DNA safe stain | To visualize the PCR products under UV light after electrophoresis on 1% agarose gel. | ||
| 7 | High Pure PCR Product Purification Kit (Roche Diagnostic GmbH) | To purify the VP1 gene PCR products according to the manufacturer's protocol. |