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1
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Phosphate buffer saline
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To make 10% stool suspension for sample processing.
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https://www.hilarispublisher.com/abstract/human-bocavirus-hbov1-and-hbov2-in-children-with-acute-gastroenteritis-from-north-india-24117.html
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View Related Publication
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2
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0.45 μm filter
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To filter the supernatant after stool suspension and centrifugation.
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3
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Cryovials
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To store the filtered supernatant at -20°C until use.
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4
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QiAamp DNA mini extraction kit (QIAGEN, Inc.)
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To extract total nucleic acids from stool filtrate.
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5
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Taq DNA polymerase (New England Biolabs, NEB)
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To perform PCR reactions.
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6
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Thermopol reaction buffer with MgCl
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To provide a buffering environment for PCR reactions.
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7
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Pan-Bocavirus NS gene primers
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To detect HBoV nucleic acids by amplifying NS gene region.
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8
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VP1/2 gene primers
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To detect HBoV nucleic acids by amplifying VP1/2 gene region.
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9
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Agarose gel (2%)
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To visualize PCR products by electrophoresis.
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10
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DNA molecular weight marker VIII
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To determine the size of PCR products on agarose gel.
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11
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Exo-Sap-IT (USB)
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To treat HBoV positive second-round PCR products before direct sequencing.
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