Panoramic Parvovirus Strain Discovery Data Chain
| ParvoDB ID | PV010879 | GenBank Accession | FJ170281 |
| Subfamily | Parvovirinae | Genus | Bocaparvovirus |
| Species | Bocaparvovirus primate2 | Sequence Length | 473 |
| Definition | Human bocavirus 2 isolate PK-2299 NS1 gene, partial cds. | Submission Date | 2008-08-28 |
| Strain Name | PK-2299 | Sampling Date | |
| Sampling Country | Pakistan | Location | Pakistan |
| Submitted By | Kapoor,A., Slikas,E., Simmonds,P., Chieochansin,T., Naeem,A., Shaukat,S., Alam,M.M., Sharif,S., Angez,M., Zaidi,S. and Delwart,E. | Submitting Institution | Molecular Virology, Blood Systems Research Institute, 270, Masonic Avenue, San Francisco, CA 94118, USA |
| Original Host | Homo sapiens | Sample Type | stool samples |
| Standardized Host Name | Homo sapiens | Host Tax Rank | species |
| Standardized Host Class | Mammalia | Standardized Host Order | Primates |
| Environmental Origin | - N/A - | Reagent Origin | - N/A - |
| Number | Reagent/Consumables | Purpose | PMID/Url | Reference |
|---|---|---|---|---|
| 1 | Stool samples from children | Collected from 57 children with non-polio acute flaccid paralysis (AFP) and 41 healthy children in Pakistan as part of the WHO poliovirus eradication program. Also, 699 stool samples from the UK for enteric bacteriology screening. | 19072716 | View Related Publication |
| 2 | Viral metagenomics processing reagents | Used to process stool supernatants for the discovery and analysis of viral-sized particles. | ||
| 3 | Random PCR products | Derived from nuclease resistant, viral-sized particles associated RNA and DNA. | ||
| 4 | Plasmid subcloning reagents | Used for subcloning the random PCR products and sequencing plasmid inserts. | ||
| 5 | tBLASTx database | For analyzing the resulting sequences against Genbank database. | ||
| 6 | RACE (rapid amplification of cDNA ends) | To amplify the viral extremities of HBoV2. | ||
| 7 | HBoV and HBoV2 PCR primers | Used for specific amplification of HBoV and HBoV2 sequences in stool samples. | ||
| 8 | Nested PCR primers targeting HBoV and HBoV2 NS region | To determine prevalence of human bocaviruses. |