Panoramic Parvovirus Strain Discovery Data Chain
| ParvoDB ID | PV010864 | GenBank Accession | JN086998 |
| Subfamily | Parvovirinae | Genus | Bocaparvovirus |
| Species | Bocaparvovirus primate1 | Sequence Length | 5319 |
| Definition | Human bocavirus strain HBoV3, complete genome. | Submission Date | 2011-06-06 |
| Strain Name | HBoV3 | Sampling Date | 2003 |
| Sampling Country | United States of America | Location | United States of America |
| Submitted By | Kapoor,A. | Submitting Institution | Columbia University, 722 West, 168 Street, New York, NY 10032, USA |
| Original Host | Homo sapiens | Sample Type | ileal biopsy material |
| Standardized Host Name | Homo sapiens | Host Tax Rank | species |
| Standardized Host Class | Mammalia | Standardized Host Order | Primates |
| Environmental Origin | - N/A - | Reagent Origin | - N/A - |
| Number | Reagent/Consumables | Purpose | PMID/Url | Reference |
|---|---|---|---|---|
| 1 | TRIzol (Invitrogen) | DNA extraction from ileal biopsy material | 21738642 | View Related Publication |
| 2 | Nanodrop ND-1000 Spectrophotometer (Nanodrop Technologies) | Determine DNA concentration and integrity | ||
| 3 | Bioanalyzer (Agilent Technologies) | Determine DNA concentration and integrity | ||
| 4 | nanogenesis free water | Used in PCR reaction mixture | ||
| 5 | PCR primers | Amplification of conserved sequences in HBoV capsid genes for nested PCR assay | ||
| 6 | HotStart Taq DNA polymerase (Qiagen) | Polymerase reaction buffer for PCR | ||
| 7 | MgCl2 | Component of the PCR reaction mixture | ||
| 8 | dNTP | Component of the PCR reaction mixture | ||
| 9 | PCR purification kit (Qiagen) | Purification of PCR products | ||
| 10 | Restriction enzyme | Digestion and re-circularization of nucleic acids for detecting the genome-integrated form |