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1
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QIAamp DNA mini kit (Qiagen, Hilden, Germany)
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For viral DNA extraction from stool samples.
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29534770
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2
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2× Emerald Amp PCR Master Mix (TaKaRa, Shiga, Japan)
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For performing polymerase chain reaction (PCR).
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3
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5′ RACE System for RACE Version 2.0 Kit (Invitrogen, Carlsbad, CA, USA)
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For rapid amplification of cDNA ends (RACE) to determine the 5′-ends of HBoV genomic DNA.
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4
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3′-Oligo (dT)-anchor-R
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For reverse transcription to synthesize cDNA to determine the 3′-end of the HBoV genomic DNA.
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5
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HiYield Gel/PCR DNA Fragments Extraction Kit (RBC, Taipei, Taiwan)
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For extraction of PCR products.
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6
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pGEM-T Easy Vectors (Promega, Madison, WI, USA)
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For cloning PCR products.
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7
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Escherichia coli DH5α competent cells (RBC)
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For transformation of cloned vectors.
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8
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Luria–Bertani agar plates (Duchefa, Haarlem, the Netherlands) containing X-gal, isopropyl-β-D-thiogalactoside, and ampicillin
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For selection of transformed E. coli clones.
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9
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Luria–Bertani broth (Duchefa)
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For incubation of selected clones.
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10
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HiYield Plasmid Mini Kit (RBC)
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For purification of plasmid DNA.
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11
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Ethidium bromide
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For staining gels in electrophoresis to visualize PCR products.
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