ParvoDB ID PV010667 GenBank Accession AB481080
Subfamily Parvovirinae Genus Bocaparvovirus
Species Bocaparvovirus primate1 Sequence Length 5299
Definition Human bocavirus DNA, complete genome, strain: JPOC07-511. Submission Date 2009-02-13
Strain Name JPOC07-511 Sampling Date from 2006-04 to 2008-10
Sampling Country Japan Location Osaka City, Japan, Japan
Submitted By Kaida,A. Submitting Institution Contact:Atsushi Kaida Osaka City Institute of Public Health and Environmental Sciences, Microbiology; 8-34, Tojo-cho, Tennoji-ku, Osaka, Osaka 543-0026, Japan
Original Host Homo sapiens Sample Type Clinical specimens
Standardized Host Name Homo sapiens Host Tax Rank species
Standardized Host Class Mammalia Standardized Host Order Primates
Environmental Origin - N/A - Reagent Origin - N/A -
Number Reagent/Consumables Purpose PMID/Url Reference
1 QIAamp Viral RNA Mini Kit (Qiagen, Valencia, CA, USA) Extraction of viral nucleic acids from the specimens. 20536724 View Related Publication
2 TaqMan Universal PCR Master Mix (Applied Biosystems, Foster City, CA, USA) For the real-time PCR method for HBoV NP-1 gene.
3 Oligonucleotide primer pairs and TaqMan probe (Sigma-Aldrich, Tokyo, Japan) Designed for the real-time PCR detection of HBoV NP-1 gene.
4 MEM supplemented with 1% FBS Maintenance of Vero and RD-18S cells for virus isolation tests.
5 MEM supplemented with 1% FBS and 1% trypsin Culture of MDCK cells for virus isolation, particularly for FLU virus infection.