Panoramic Parvovirus Strain Discovery Data Chain

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ParvoDB ID PV010660 GenBank Accession DQ652177
Subfamily Parvovirinae Genus Bocaparvovirus
Species Bocaparvovirus primate1 Sequence Length 606
Definition Human bocavirus strain NH4032 VP1 (VP1) and VP2 (VP2) genes, partial cds. Submission Date 2006-05-25
Strain Name NH4032 Sampling Date from 2003-11 to 2004-10
Sampling Country United States of America Location United States of America
Submitted By Kahn,J.S., Kesebir,D., Vazquez,M., Weibel,C., Shapiro,E.D., Ferguson,D. and Landry,M.L. Submitting Institution Pediatrics, Yale University School of Medicine, P. O. Box 208064, New Haven, CT 06520-8064, USA
Original Host Homo sapiens Sample Type respiratory specimens
Standardized Host Name Homo sapiens Host Tax Rank species
Standardized Host Class Mammalia Standardized Host Order Primates
Environmental Origin - N/A - Reagent Origin - N/A -
Number Reagent/Consumables Purpose PMID/Url Reference
1 Viral freezing media Used to store respiratory specimens at -20 C 17041854 View Related Publication
2 Dulbecco’s modified Eagle medium Component of viral freezing media
3 MgSO4 (200 mmol/L) Component of viral freezing media
4 HEPES [pH 7.5] (100 mmol/L) Component of viral freezing media
5 QIAamp nucleic acid purification kits (Qiagen) Used for nucleic acid extraction from respiratory specimens
6 HotStar Taq polymerase (Qiagen) Used for PCR amplification
7 Forward primer 5′-GACCTCTGTAAGTACTATTAC-3′ Used for amplification of the HBoV NP-1 gene in PCR screening
8 Reverse primer 5′-CTCTGTGTTGACTGAATACAG-3′ Used for amplification of the HBoV NP-1 gene in PCR screening
9 Forward primer 5′-GGACCACAGTCATCAGAC-3′ Used for amplification and sequencing of VP1/VP2 genes
10 Reverse primer 5′-CCACTACCATCGGGCTG-3′ Used for amplification and sequencing of VP1/VP2 genes
11 Reverse-transcriptase Used for screening each HBoV-positive specimen for human metapneumovirus
12 Applied Biosystems 3730 XL DNA Analyzer Used for DNA sequencing