Panoramic Parvovirus Strain Discovery Data Chain

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ParvoDB ID PV010450 GenBank Accession MN887272
Subfamily Parvovirinae Genus Bocaparvovirus
Species Bocaparvovirus primate1 Sequence Length 5217
Definition Human bocavirus isolate HBov_JX2086 NS1, NP1, VP1, and VP2 genes, complete cds. Submission Date 2019-12-30
Strain Name HBov_JX2086 Sampling Date from 2011-08 to 2015-12
Sampling Country China Location Jiangxi, China
Submitted By Jianxiong,L., Gang,X., Yong,S., Tian,G., Jun,Z. and Ying,X. Submitting Institution Jiangxi Center for Disease Control and Prevention, beijing east road, Nanchang, State / Province 330029, China
Original Host Homo sapiens Sample Type Throat swab or broncholavage fluid
Standardized Host Name Homo sapiens Host Tax Rank species
Standardized Host Class Mammalia Standardized Host Order Primates
Environmental Origin - N/A - Reagent Origin - N/A -
Number Reagent/Consumables Purpose PMID/Url Reference
1 Respiratory specimens Collected from children with respiratory tract infection for Human Bocavirus positive sample sequencing and analysis. https://kns.cnki.net/kcms2/article/abstract?v=BS8_DD2Uwa7J-uk6TvJ6TVoZXLgF45v_3lW1d9qR6XwdhO6kRL-Zb80IxeBoxuNpd_YAZfrKW3LFkZm8e0oyBPc3wTs_4203WUAKNO2zBRpbmgBZUkq8yWwL2ps-5Xa82ZoyWrwjuFw=&uniplatform=NZKPT&flag=copy View Related Publication
2 Bio-Rad PCR instrument Used for PCR amplification of Human Bocavirus samples.
3 VILBER LOURMAT gel imaging system Used for imaging and analyzing gel electrophoresis results.
4 Qiagen DNA mini kit For extracting total viral nucleic acid from the specimens.
5 Promaga GoTaq Master Mix kit Used for RT-PCR reaction to amplify Human Bocavirus positive samples.
6 1.5% agarose gel For separating and recovering positive Human Bocavirus gene PCR amplification products.
7 pGM-T plasmid Used to ligate the target DNA fragment recovered from the agarose gel and transform it into E. coli DH5A competent cells.
8 E. coli DH5A competent cells Used to transform the ligated recombinant plasmid and select positive clones.
9 ST1 (DQ000495.1) Standard strain downloaded from GenBank for sequence analysis and comparison.
10 ST2 (DQ000496.1) Standard strain downloaded from GenBank for sequence analysis and comparison.
11 CU74 (EF203922.1) Standard strain downloaded from GenBank for sequence analysis and comparison.
12 WLL-1 (DQ778300.1) Standard strain downloaded from GenBank for sequence analysis and comparison.
13 SH2 (FJ375128.1) Standard strain downloaded from GenBank for sequence analysis and comparison.
14 CQ53 (JX434059.1) Standard strain downloaded from GenBank for sequence analysis and comparison.
15 BJ3064 (DQ988933.1) Standard strain downloaded from GenBank for sequence analysis and comparison.
16 HZ1403 (KP710213.1) Standard strain downloaded from GenBank for sequence analysis and comparison.
17 FZ40 (GQ455987) Standard strain downloaded from GenBank for sequence analysis and comparison.
18 HK24 (EF450740.1) Standard strain downloaded from GenBank for sequence analysis and comparison.
19 ZJ92 (JX887482.1) Standard strain downloaded from GenBank for sequence analysis and comparison.
20 GZ9081 (JN794566.1) Standard strain downloaded from GenBank for sequence analysis and comparison.