Panoramic Parvovirus Strain Discovery Data Chain
| ParvoDB ID | PV010061 | GenBank Accession | KR819815 |
| Subfamily | Parvovirinae | Genus | Erythroparvovirus |
| Species | Erythroparvovirus primate1 | Sequence Length | 994 |
| Definition | Human parvovirus B19 isolate A27 NS1 and VP1u genes, partial cds. | Submission Date | 2015-05-13 |
| Strain Name | A27 | Sampling Date | 2009 |
| Sampling Country | China | Location | China |
| Submitted By | Jia,J., Ma,Y. and Zhao,X. | Submitting Institution | Laboratory for Viral Safety of National Centre of Biomedical Analysis, Beijing Institute of Transfusion Medicine, No. 27 Taiping Road, Beijing 100080, China |
| Original Host | Homo sapiens | Sample Type | plasma pool samples |
| Standardized Host Name | Homo sapiens | Host Tax Rank | species |
| Standardized Host Class | Mammalia | Standardized Host Order | Primates |
| Environmental Origin | - N/A - | Reagent Origin | - N/A - |
| Number | Reagent/Consumables | Purpose | PMID/Url | Reference |
|---|---|---|---|---|
| 1 | High Pure Viral Nucleic Acid Kit (Roche Diagnostics, Mannheim, Germany) | DNA extraction from the source plasma pool samples. | 27639978 | View Related Publication |
| 2 | Taq DNA polymerase and dNTPs from Qiagen (Germany) | Used for PCR amplification. | ||
| 3 | 1% agarose gels from Biotec (China) | Electrophoresis and analysis of PCR products. | ||
| 4 | TIANgel Midi Purification Kit (Tiangen Biotech, Beijing, China) | Purification of PCR products. | ||
| 5 | pMD18-T vector (TaKaRa Bio, Dalian, China) | Cloning of PCR products. | ||
| 6 | BigDye Terminator v3.1 Cycle Sequencing Kit (Applied Biosystems, Foster City, USA) | Sequencing DNA. | ||
| 7 | Recombinant enzymes (New England Biolabs, Ipswich, MA, USA) | Restriction digestion for verification of clones. | ||
| 8 | Competent cells (Tiangen Biotech, Beijing, China) | Transformation with vector-cloned PCR products. |