Panoramic Parvovirus Strain Discovery Data Chain

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ParvoDB ID PV009726 GenBank Accession MH884867
Subfamily Parvovirinae Genus Bocaparvovirus
Species Bocaparvovirus primate1 Sequence Length 203
Definition UNVERIFIED: Human bocavirus isolate KK-NA147-15/th capsid protein-like gene, partial sequence. Submission Date 2018-09-05
Strain Name KK-NA147-15/th Sampling Date 2015-11-01
Sampling Country Thailand Location Srinagarind Hospital or Khon, Thailand
Submitted By Jantawong,C., Prariyachatigul,C., Pinlaor,P., Teeratakulpisarn,J., Srinakarin,J. and Pakarasang,M. Submitting Institution Biomedical Science Program, Graduate School, Khon Kaen University, 123 Mittraphap Road, Muang, Khon Kaen 40002, Thailand
Original Host Homo sapiens Sample Type Nasopharyngeal aspirate,nasopharyngeal swab,blood and feces
Standardized Host Name Homo sapiens Host Tax Rank species
Standardized Host Class Mammalia Standardized Host Order Primates
Environmental Origin - N/A - Reagent Origin - N/A -
Number Reagent/Consumables Purpose PMID/Url Reference
1 Phosphate-buffered saline (PBS, pH 7.2) Used in nasopharyngeal swab collection to maintain the neutral pH of samples. https://www.tm.mahidol.ac.th/seameo/2018-49-5/09-7622-20-805.pdf View Related Publication
2 EDTA tubes Used for collecting blood samples to prevent coagulation.
3 Sterile normal saline Used to prepare 10% suspensions of fecal samples.
4 QIAamp MinElute Virus Spin Kit (QIAGEN) Used for extracting viral nucleic acid from nasopharyngeal aspirate (NPA), nasopharyngeal swab (NPS), and plasma samples.
5 QIAamp Viral RNA Mini Kit (QIAGEN) Used for extracting viral RNA from the fecal sample suspensions.
6 Nested-PCR primers Used for detecting HBoV DNA in samples. Primer sequences are specific for human bocavirus, targeting the VP1 region.
7 Taq DNA polymerase (NEB) Enzyme used in PCR to amplify HBoV DNA.
8 dNTPs Nucleotide mixture used in PCR reactions for DNA synthesis.
9 Thermopol reaction buffer (NEB) Buffer solution used to provide optimal conditions for PCR.
10 Ethidium bromide Used to stain DNA in agarose gels for visualization under UV light.
11 Geneaid Gene pHlow Gel/PCR Kit Used for purification of PCR amplicons from agarose gels.
12 DNA sequencing service (Macrogen) Used for sequencing purified PCR amplicons for phylogenetic analysis.