Panoramic Parvovirus Strain Discovery Data Chain
| ParvoDB ID | PV009645 | GenBank Accession | KF664145 |
| Subfamily | Parvovirinae | Genus | Erythroparvovirus |
| Species | Erythroparvovirus primate1 | Sequence Length | 812 |
| Definition | UNVERIFIED: Human parvovirus B19 isolate L4805 genomic sequence. | Submission Date | 2013-09-12 |
| Strain Name | L4805 | Sampling Date | 2012-09-01 |
| Sampling Country | India | Location | North India, India |
| Submitted By | Jain,A., Prakash,S., Khan,D.N., Jain,P., Singh,D.D. and Singh,K.P. | Submitting Institution | Microbiology, King George Medical University, Shahmeena Road, Chowk, Lucknow, Uttar Pradesh 226003, India |
| Original Host | Homo sapiens | Sample Type | blood samples |
| Standardized Host Name | Homo sapiens | Host Tax Rank | species |
| Standardized Host Class | Mammalia | Standardized Host Order | Primates |
| Environmental Origin | - N/A - | Reagent Origin | - N/A - |
| Number | Reagent/Consumables | Purpose | PMID/Url | Reference |
|---|---|---|---|---|
| 1 | ELISA kits (NovaTec Immunodiagnostica, Dietzenbach, Germany) | Detection of anti-B19V IgM and anti-B19V IgG antibodies. | 25087946 | View Related Publication |
| 2 | DNA extraction kits (Invitrogen, Carlsbad, CA) | Extraction of DNA from blood samples. | ||
| 3 | Primer pairs for nested PCR | First and second round PCR amplification targeting the VP1/VP2 capsid protein genes for B19V DNA detection. | ||
| 4 | Quantitative real-time PCR reagents | Estimation of B19V viral load in PCR positive cases. | ||
| 5 | PureLink Quick Gel Extraction kit (Invitrogen) | Purification of PCR products for sequencing. | ||
| 6 | BigDye Terminator cycle sequencing mix (Applied Biosystems, Foster City, CA) | Sequencing of purified amplicons. |