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1
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Commercial ELISA kits (NovaLisaTM, NovaTech Immunodiagnostica, Dietzenbach, Germany)
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Used for testing serum samples for the presence of Parvovirus B19 specific IgM & IgG antibodies.
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29170063
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View Related Publication
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2
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Probes and primers designed in house
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Detection of B19V-DNA using real-time PCR.
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3
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PureLink Viral RNA/DNA extraction Kit (Invitrogen, Life Technologies, CA, USA)
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Extraction of DNA from serum samples.
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4
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Molecular grade water
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Used as no template control (NTC) during DNA extraction and PCR.
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5
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B19V negative serum controls (NC) and B19V positive serum controls (PC)
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To ensure accuracy and reliability of PCR results.
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6
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Filter tips
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Prevent laboratory cross-contamination during molecular techniques.
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7
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AgPath ID (Ambion)
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Components of the PCR master mix for real-time PCR.
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8
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GeneJET Plasmid Miniprep Kit (K0502, Fermentas)
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Extraction of plasmids from bacteria.
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9
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TA Cloning Kit (Fermentas)
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Ligation of PCR products into pTZ57R/T cloning vector.
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10
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E. coli DH5α strain
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Transformation with plasmid ligated product.
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11
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ABI PRISM BigDye Terminator Cycle Sequencing Ready Reaction Kit (version 3.1)
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Sequencing the PCR amplified products.
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