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1
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Sterile physiological saline fluid
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Collection of nasopharyngeal secretions
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18644746
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View Related Publication
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2
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Disposable mucus extractor
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Collection of nasopharyngeal secretions
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3
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Monoclonal antibodies (Argene Biosoft, Varhiles, France)
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Immunofluorescence assays for viral antigen detection
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4
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Standard viral transport medium
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Dilution of nasopharyngeal secretion specimen
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5
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Continuous human diploid fibroblasts (MRC-5) and Rhesus monkey kidney (MA-104) cells
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Viral cell-culture detection
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6
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Immunofluorescence antigen detection assays reagents
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Typing of virus isolates in infected cell monolayers
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7
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High Pure Viral Nucleic Acid Kit (Roche Diagnostics, Mannheim, Germany)
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Extraction of total nucleic acids
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8
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Diethyl-pyrocarbonate (DEPC) sterile water
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Elution and storage of nucleic acids
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9
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iQTMSYBR Green Supermix 2X (Bio-Rad, Marnes-la-coquette, France)
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Real-time PCR reactions
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10
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Primers HBoV-F (TATGGCCAAGGCAATCGTCCAAG) and HBoV-R (GCCGCCTGAACATGAGAAACAGA)
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Ampli?cation of a 291-bp fragment of HBoV NS-1 gene in real-time PCR
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11
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TaqMan GAPDH Control Reagents (Applied Biosystem, Courtaboeuf, France)
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Quantitative detection of GAPDH for normalization in real-time PCR
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12
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Primers for VP1/VP2 genes and PCR amplification reagents
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Amplification of a 404-bp fragment of HBoV VP1 and VP2 genes
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13
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DNA sequencing reagents (Applied Biosystems) and ABI 3130 Sequencer
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Sequencing of VP1/VP2 gene PCR products
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