Panoramic Parvovirus Strain Discovery Data Chain
| ParvoDB ID | PV009500 | GenBank Accession | LN680954 |
| Subfamily | Parvovirinae | Genus | Erythroparvovirus |
| Species | Erythroparvovirus primate1 | Sequence Length | 985 |
| Definition | Human parvovirus B19 partial NS1 gene for NS1 protein, strain B19Vs/Sofia.BGR/12.06. | Submission Date | 2014-11-21 |
| Strain Name | B19Vs/Sofia.BGR/12.06 | Sampling Date | 2006 |
| Sampling Country | Bulgaria | Location | Sofia, Bulgaria |
| Submitted By | Ivanova,S.K., Mihneva,Z.G., Toshev,A.K., Kovaleva,V.P., Andonova,L.G., Muller,C.P. and Huebschen,J.M. | Submitting Institution | Sante, CRP-Sante / Laboratoire National de, Institute of Immunology, 20A, rue Auguste Lumiere, L-1950 Luxembourg, LUXEMBOURG |
| Original Host | None | Sample Type | serum samples |
| Standardized Host Name | Homo sapiens | Host Tax Rank | species |
| Standardized Host Class | Mammalia | Standardized Host Order | Primates |
| Environmental Origin | - N/A - | Reagent Origin | - N/A - |
| Number | Reagent/Consumables | Purpose | PMID/Url | Reference |
|---|---|---|---|---|
| 1 | Mikrogen, recomWell Parvovirus B19 IgM | Commercial indirect enzyme-linked immunosorbent assay (ELISA) used to test for parvovirus B19 IgM antibodies in serum samples. | 26847955 | View Related Publication |
| 2 | NucleoSpin Blood test kits (Macherey-Nagel GmbH & Co. KG) | Used for viral DNA extraction from IgM positive serum samples. | ||
| 3 | KAPA Taq PCR kits (Kapa Biosystems, Inc.) | Used for polymerase chain reaction (PCR) amplification targeting the NS1 gene region of B19V DNA. | ||
| 4 | Ethidium bromide | Used to stain agarose gels for electrophoresis and visualization of PCR products. | ||
| 5 | QIAquick PCR purification kits (Qiagen) | Used to purify PCR products for sequencing. | ||
| 6 | BigDye Terminator v3.1 cycle sequencing kit (Life Technologies) | Used for sequencing of the NS1/VP1u PCR positive samples. |