ParvoDB-Parvovirus Database

ParvoDB ID	PV009427	GenBank Accession	AB030689
Subfamily	Parvovirinae	Genus	Erythroparvovirus
Species	Erythroparvovirus primate1	Sequence Length	424
Definition	Human parvovirus B19 NS1 gene for non-structural protein NS1, partial cds, isolate: N2.	Submission Date	1999-07-29
Strain Name	N2	Sampling Date	1981-12
Sampling Country	Japan	Location	Fukuoka, Japan
Submitted By	Ishii,K.K.	Submitting Institution	Contact:Keiko K Ishii Tohoku University School of Medicine, Department of Molecular Diagnostics; 1-1 Seiryo-machi, Aoba-ku, Sendai, Miyagi 980-8574, Japan

Number	Reagent/Consumables	Purpose	PMID/Url	Reference
1	Escherichia coli DNA polymerase I Klenow fragment	To synthesize double-stranded DNA from single-stranded B19 DNAs utilizing the hairpin structure at the 3' end of each molecule.	2157807	View Related Publication
2	AatII	To cleave the terminal repeats of the B19 genome.
3	Nuclease S1	To remove the terminal 'turn around' form of B19 DNAs.
4	Klenow fragment (second usage)	To convert the staggered ends of B19 DNAs to blunt ends.
5	Vector plasmid DNAs of pUC18	To join with B19 DNA converted to blunt ends at the SmaI site.
6	Escherichia coli JM83	For transformation with the ligated mixture containing vector plasmid DNAs of pUC18 and B19 DNA.
7	Restriction endonucleases (REs): BamHI, BgllI, BstEII, EcoRI, HindIII, HpaI, KpnI, PstI, PvuII, SalI, SmaI, XbaI, and XhoI	To analyze the structures of the B19 DNAs by cleavage.
8	Ethidium bromide	To stain cleaved DNAs in agarose gels for visualization.
9	Nylon membranes	To transfer DNAs from agarose gels for hybridization.
10	32P-labelled probe (0.7 kb PvuII-EcoRI fragment of pJB)	To hybridize with Bglll and PvuII cleaved DNAs.
11	32P-labelled probe (0.90 kb HindIII-HindIII fragment of pJB)	To hybridize with HindIII digests.

Panoramic Parvovirus Strain Discovery Data Chain