Panoramic Parvovirus Strain Discovery Data Chain

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ParvoDB ID PV009425 GenBank Accession AB030673
Subfamily Parvovirinae Genus Erythroparvovirus
Species Erythroparvovirus primate1 Sequence Length 4628
Definition Human parvovirus B19 NS1, VP1, VP2 genes for non-structural protein NS1, capsid protein VP1, capsid protein VP2, complete cds, isolate: N8. Submission Date 1999-07-29
Strain Name N8 Sampling Date 1986-12
Sampling Country Japan Location Tokyo, Japan
Submitted By Ishii,K.K. Submitting Institution Contact:Keiko K Ishii Tohoku University School of Medicine, Department of Molecular Diagnostics; 1-1 Seiryo-machi, Aoba-ku, Sendai, Miyagi 980-8574, Japan
Original Host None Sample Type serum samples
Standardized Host Name Homo sapiens Host Tax Rank species
Standardized Host Class Mammalia Standardized Host Order Primates
Environmental Origin - N/A - Reagent Origin - N/A -
Number Reagent/Consumables Purpose PMID/Url Reference
1 Escherichia coli DNA polymerase I Klenow fragment To synthesize double-stranded DNA from single-stranded B19 DNAs utilizing the hairpin structure at the 3' end of each molecule. 2157807 View Related Publication
2 AatII To cleave the terminal repeats of the B19 genome.
3 Nuclease S1 To remove the terminal 'turn around' form of B19 DNAs.
4 Klenow fragment (second usage) To convert the staggered ends of B19 DNAs to blunt ends.
5 Vector plasmid DNAs of pUC18 To join with B19 DNA converted to blunt ends at the SmaI site.
6 Escherichia coli JM83 For transformation with the ligated mixture containing vector plasmid DNAs of pUC18 and B19 DNA.
7 Restriction endonucleases (REs): BamHI, BgllI, BstEII, EcoRI, HindIII, HpaI, KpnI, PstI, PvuII, SalI, SmaI, XbaI, and XhoI To analyze the structures of the B19 DNAs by cleavage.
8 Ethidium bromide To stain cleaved DNAs in agarose gels for visualization.
9 Nylon membranes To transfer DNAs from agarose gels for hybridization.
10 32P-labelled probe (0.7 kb PvuII-EcoRI fragment of pJB) To hybridize with Bglll and PvuII cleaved DNAs.
11 32P-labelled probe (0.90 kb HindIII-HindIII fragment of pJB) To hybridize with HindIII digests.