1
Collagenase type I
To treat synovial tissue and facilitate the release of synovial cells.
15383603
2
Proteinase K
To extract DNA from synovial cells.
3
Phenol-chloroform
To purify DNA during extraction from synovial cells.
4
PCR Primer Set (5'-TTCCCGCCTTATGCAAATGGGCAG-3' and 5'-GTGTTAGGCTGTCTTATAGGTAC-3')
To amplify almost the whole B19 genome including coding regions NS1, VP1, and VP2.
5
pGEM-T vector
To clone the amplified B19 genome fragment for sequencing.
6
7-deaza-dGTP cycle sequencing kit
For sequencing the cloned B19 genome.
7
PCR Primer Set (5'-CGCCTGGAACACTGAAACCC-3' and 5'-AGCCTGCACCTGAGGAGTGA-3')
To amplify the NS1 region directly from synovial DNA samples.
8
Calf type II collagen (CII)
For immunization of mice to induce collagen-induced arthritis.
9
CFA (Complete Freund's Adjuvant) containing Mycobacterium tuberculosis strain H37 Rv
To emulsify CII for initial immunization at the tail base.
10
IFA (Incomplete Freund's Adjuvant)
To emulsify CII for the booster immunization of mice.
11
ISOGEN reagent
For total RNA isolation from mouse tissues.
12
RT-PCR (Reverse Transcription-PCR) kit Ver. 2.1
To detect NS1 mRNA in tissue samples.
13
10% Neutral buffered formalin
To fix tissues for histopathological examination.
14
5% EDTA-2Na solution
To decalcify fixed joint tissues.
15
H&E (Hematoxylin and Eosin)
To stain tissue sections for histopathological examination.
16
Human monoclonal anti-NS1 Ab
For immunohistochemical staining to detect NS1 protein in tissues.
17
Human monoclonal anti-VP1 Ab
For immunohistochemical staining to detect VP1 protein in tissues.
18
Biotinylated anti-human IgG Fc fragment
To detect human monoclonal antibodies in immunohistochemical staining.
19
Streptavidin
To detect biotin-labeled antibodies in immunohistochemical staining.
20
3,3'-diaminobenzidine (DAB)
To visualize peroxidase activity in immunohistochemical staining.
21
ELISA plates
For measuring serum TNF-α concentration and anti-CII antibody titers.
22
Goat anti-mouse IgG with HRP
To detect mouse antibodies in ELISA.
23
o-phenylendiamine and H2O2
To develop color in ELISA for detecting bound antibodies.
24
[3H]Thymidine
To measure cell proliferation response in a tritiated thymidine incorporation assay.
