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1
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guanidinium buffer and silica
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Nucleic acids extraction from 100 ll of NPA or NPS specimens.
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25269520
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View Related Publication
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2
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Platinum Taq DNA polymerase (Invitrogen)
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Used for amplification in real-time PCR (Applied Biosystems 7500 Real-Time PCR System) of the NP1 gene for relative quantification of HBoV1.
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3
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SYBR Green (Invitrogen S-7563)
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Added to the amplification reaction mixture for detection in real-time PCR.
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4
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AgPath-IDTM One-Step RT-PCR Reagents (Applied Biosystems)
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qRT-PCR for detection of other viral pathogens including Flu A and B viruses, PIV1, -2 and -3, AdV, RSV and MPV in HBoV-positive samples.
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5
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dual-labeled FAM-BHQ1 probes
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Used in PCR assay as part of the detection system for various respiratory pathogens.
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6
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QIAGEN PCR cleanup kit
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Purification of PCR products prior to sequencing.
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7
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ABI PRISM BigDye Terminator v. 3.1 (PE Applied Biosystems)
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Used for sequencing reactions of amplified NP1 and VP1 regions.
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8
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ethidium bromide
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Staining of agarose gels to resolve PCR products visually.
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