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1
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Murine norovirus (MNV1)
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Seeded in wastewater samples as a sample process control.
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27311692
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2
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Glycine pH 9.5 (2.5 M)
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Treated wastewater samples to facilitate virus concentration.
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3
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Chloroform
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Used to treat samples and facilitate viral concentration.
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4
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NucliSENS easyMAG (BioMerieux, Marcy l’Etoile, France) semi-automated extraction system with magnetic silica
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Used for viral nucleic acid extraction from chloroform-treated samples.
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5
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MyTaqTM Red Mix PCR kit (Bioline, London, UK)
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Provided reagents for performing nested PCR assays.
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6
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Montage PCRm96 Micro-well Filter Plate (Millipore, Billerica, Mass)
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Used for purification of PCR products.
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7
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pGEM-T Vector Systems (Promega)
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Used for cloning PCR amplicons in pGEM-T vector.
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8
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NEB Turbo Competent E. coli (BioLabs inc)
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Used for transformation during cloning of PCR amplicons.
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9
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One-step FastPlasmid mini purification kit (5Prime.com)
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Used for plasmid DNA extraction from grown colonies.
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10
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ABI files
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Obtained by sequencing, aligned, and assembled into consensus sequences using software.
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11
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SensiMixT II Probe No-ROX Kit (Bioline)
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Used for real-time PCR reactions.
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12
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Ethidium bromide-stained agarose gel
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Quantified DNA plasmids during plasmid preparation.
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13
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Primers and probe targeting the VP1/VP2 gene
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Designed for real-time PCR assay.
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