ParvoDB ID PV009176 GenBank Accession FN295728
Subfamily Parvovirinae Genus Erythroparvovirus
Species Erythroparvovirus primate1 Sequence Length 994
Definition Human Parvovirus B19 partial NS1 gene and partial VP1u gene, strain RUS10. Submission Date 2009-03-23
Strain Name RUS10 Sampling Date from 2006 to 2007
Sampling Country Russia Location Russia
Submitted By Huebschen,J.M. Submitting Institution Huebschen J.M., Laboratoire National de Sante, Institute of Immunology, 20A, rue Auguste Lumiere, L-1950 Luxembourg, LUXEMBOURG
Original Host Homo sapiens Sample Type Serum samples
Standardized Host Name Homo sapiens Host Tax Rank species
Standardized Host Class Mammalia Standardized Host Order Primates
Environmental Origin - N/A - Reagent Origin - N/A -
Number Reagent/Consumables Purpose PMID/Url Reference
1 Forward primers e1855f and e1863f Used for the nested PCR. 19741071 View Related Publication
2 Reverse primers B19-R1 (5'-GGGAACTTCCGGCAAACTTCCTTG-3') and B19-R2 (5'-GTAGTCTTTTACTACTTGTGCTTG-3') Yielding fragments of 1,239 and 1,168 nt in the nested PCR.
3 Previously published reverse primers e2953r and e2960r Compared with new reverse primers to evaluate mismatches in primer binding sites.
4 New reverse primers Designed to have no more than two mismatches with B19V sequences, used for more sensitive amplification of the NS1/VP1-unique region junction.