ParvoDB ID PV009026 GenBank Accession FN295578
Subfamily Parvovirinae Genus Erythroparvovirus
Species Erythroparvovirus primate1 Sequence Length 994
Definition Human Parvovirus B19 partial NS1 gene and partial VP1u gene, strain BGR-Gabriela. Submission Date 2009-03-23
Strain Name BGR-Gabriela Sampling Date from 2005 to 2007
Sampling Country Bulgaria Location Bulgaria
Submitted By Huebschen,J.M. Submitting Institution Huebschen J.M., Laboratoire National de Sante, Institute of Immunology, 20A, rue Auguste Lumiere, L-1950 Luxembourg, LUXEMBOURG
Original Host Homo sapiens Sample Type Serum samples
Standardized Host Name Homo sapiens Host Tax Rank species
Standardized Host Class Mammalia Standardized Host Order Primates
Environmental Origin - N/A - Reagent Origin - N/A -
Number Reagent/Consumables Purpose PMID/Url Reference
1 Forward primers e1855f and e1863f Used for the nested PCR. 19741071 View Related Publication
2 Reverse primers B19-R1 (5'-GGGAACTTCCGGCAAACTTCCTTG-3') and B19-R2 (5'-GTAGTCTTTTACTACTTGTGCTTG-3') Yielding fragments of 1,239 and 1,168 nt in the nested PCR.
3 Previously published reverse primers e2953r and e2960r Compared with new reverse primers to evaluate mismatches in primer binding sites.
4 New reverse primers Designed to have no more than two mismatches with B19V sequences, used for more sensitive amplification of the NS1/VP1-unique region junction.