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1
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Viral DNA extract from nasopharyngeal aspirate
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Used to amplify the HBoV1 genome and terminal hairpins.
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22956907
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2
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Phusion high fidelity PCR kit (NEB)
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Used to amplify the left-end hairpin (LEH) and the right-end hairpin (REH) of HBoV1.
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3
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QIAquick gel extraction kit (Qiagen)
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Used to extract PCR-generated DNA.
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4
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pGEM-T vector (Promega)
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Used for cloning the PCR-generated DNA.
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5
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Sure 2 (Agilent)
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Escherichia coli strain used for all cloning work.
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6
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pProEX HTb vector (Invitrogen)
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Vector backbone for construction of pBBSmaI.
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7
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Lipofectamine and Plus reagents (Invitrogen/Life Technologies)
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Used for transfection of plasmids into cells.
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8
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Ad5 at an MOI of 5
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Used to study the effect of adenovirus helper function on pIHBoV1 replication.
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9
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pHelper plasmid (Agilent)
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Contains Ad5 E2a, E4orf6, and VA genes; used in cotransfection to support HBoV1 replication.
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10
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SDS-8% polyacrylamide gel
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Used for Western blot analysis.
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11
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Anti-NS1, Anti-NP1, Anti-VP1/2 antibodies
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Used in immunofluorescence and Western blot analysis for detecting HBoV1 proteins.
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12
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Benzonase (Sigma)
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Used to treat collected virus samples to degrade nucleic acids.
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13
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Proteinase K
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Used to digest proteins in virus samples.
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14
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QIAamp blood mini kit (Qiagen)
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Used to extract viral DNA from digested virus samples.
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15
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PrimeTime dual-labeled probe (IDT Inc.)
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Used in qPCR for quantification of HBoV1 DNA.
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16
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Premix Ex Taq (Takara Bio USA)
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Used for qPCR amplification.
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17
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3.7% paraformaldehyde in PBS
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Used to fix HAE membranes for immunofluorescence analysis.
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18
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4% paraformaldehyde
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Used to fix HAE membranes for histology analysis.
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19
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Hematoxylin and eosin (H&E)
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Used to stain HAE membrane sections for histology analysis.
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