Panoramic Parvovirus Strain Discovery Data Chain

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ParvoDB ID PV008737 GenBank Accession MT496778
Subfamily Parvovirinae Genus Dependoparvovirus
Species Dependoparvovirus primate1 Sequence Length 2208
Definition Adeno-associated virus isolate AAVv66 capsid protein VP1 gene, complete cds. Submission Date 2020-05-17
Strain Name AAVv66 Sampling Date
Sampling Country China Location China
Submitted By Hsu,H.-L., Brown,A., Loveland,A.B., Lotun,A., Xu,M., Luo,L., Xu,G., Li,J., Ren,L., Su,Q., Gessler,D.J., Wei,Y., Tai,P.W.L., Korostelev,A. and Gao,G. Submitting Institution Horae Gene Therapy Center, University of Massachusetts Medical School, 368 Plantation St., Worcester, MA 01605, USA
Original Host Homo sapiens Sample Type tissue
Standardized Host Name Homo sapiens Host Tax Rank species
Standardized Host Class Mammalia Standardized Host Order Primates
Environmental Origin - N/A - Reagent Origin - N/A -
Number Reagent/Consumables Purpose PMID/Url Reference
1 B1 antibody Recognizes C-terminal residues of the AAV VPs (except AAV4) in denatured samples. 37310260 View Related Publication
2 A20 antibody Identifies epitopes of the AAV2 which are only available when the capsid is in its native conformation.
3 C37 antibody Identifies epitopes of the AAV2 which are only available when the capsid is in its native conformation.
4 1F antibody Recognizes all the AAV2 replication proteins in both native and denatured conformations.
5 HEK 293 cells Used for the expression and production of recombinant wtAAV2 and AAV2 variants.
6 pXRAAV2 plasmid Contains the AAV2 VP1, -2, and -3 sequences, and AAV2 replication proteins, used to generate selected rAAV2 mutants.
7 pHelper Contains the adenovirus helper genes, used in the triple transfection for AAV production.
8 pTR-UF3-luciferase Contains the reporter gene and the AAV inverted terminal repeats required for genome packaging.
9 Polyethylene glycol (PEG) Used for the precipitation of viral particles.
10 Benzonase Removes all the nucleic acid that was not encapsidated.
11 Elution buffer (0.1 M glycine-HCl, pH 2.7) Used to elute the virus from the AVB Sepharose HP column.
12 Neutralization buffer (1 M Tris-HCl, pH 10) Used to neutralize the eluted virus solution.
13 SYBR green supermix Contains Taq DNA polymerase, used in qPCR for determining genome titer.
14 Luciferase assay kit Used to measure luminescence for determining the infectivity of the AAV vectors.
15 Uranyl acetate Used for negative-staining in electron microscopy to visualize viral capsids.
16 SYPRO orange Used in differential scanning fluorimetry (DSF) to monitor binding to exposed hydrophobic regions during capsid denaturation.