Panoramic Parvovirus Strain Discovery Data Chain

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ParvoDB ID PV008694 GenBank Accession MG195156
Subfamily Parvovirinae Genus Bocaparvovirus
Species Bocaparvovirus primate2 Sequence Length 5107
Definition Human bocavirus strain CUK18, partial genome. Submission Date 2017-10-12
Strain Name CUK18 Sampling Date 2013-07-16
Sampling Country South Korea Location Gyeonggido, South Korea
Submitted By Hong,S.-J. and Paik,S.-Y. Submitting Institution Department of Biomedical Sciences, College of Medicine, The Catholic University of Korea, 222 Banpo-daero Seocho-gu, Seoul, Seoul 137-701, Korea
Original Host Homo sapiens Sample Type Fecal samples
Standardized Host Name Homo sapiens Host Tax Rank species
Standardized Host Class Mammalia Standardized Host Order Primates
Environmental Origin - N/A - Reagent Origin - N/A -
Number Reagent/Consumables Purpose PMID/Url Reference
1 QIAamp DNA Mini kit (Qiagen, Hilden, Germany) Extraction of viral genomic DNA from 140 μL of 10% fecal suspension https://brieflands.com/articles/jjm-79145 View Related Publication
2 2 × Emerald Amp PCR Master Mix (TaKaRa, Shiga, Japan) PCR amplification of HBoV genomic DNA
3 RACE version 2.0 kit (Invitrogen, Carlsbad, CA, USA) Determination of 5' and 3' terminal sequences of the HBoV genome
4 HiYield Gel/PCR DNA Fragments Extraction kit (RBC, Taipei, Taiwan) Purification of amplified fragments
5 pGEM-T Easy Vectors (Promega, Madison, WI, USA) Cloning of purified PCR products
6 Luria-Bertani agar plates (Duchefa, Haarlem, Netherlands) containing 40 ng/mL X-gal, 0.1 mM isopropyl-β-D-thiogalactoside, and 50 mg/mL ampicillin Selection of transformants
7 HiYield Plasmid Mini Kit (RBC, Taipei, Taiwan) Purification of plasmid DNA
8 Ethidium bromide Visualization of DNA in agarose gel electrophoresis