Panoramic Parvovirus Strain Discovery Data Chain

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ParvoDB ID PV008692 GenBank Accession AY044266
Subfamily Parvovirinae Genus Erythroparvovirus
Species Erythroparvovirus primate1 Sequence Length 4935
Definition Human parvovirus B19 isolate LaLi non-structural protein NS1 (NS1), minor virus capsid protein VP1 (VP1), and major virus capsid protein VP2 (VP2) genes, complete cds. Submission Date 2006-11-15
Strain Name LaLi Sampling Date
Sampling Country None Location None
Submitted By Hokynar,K., Soderlund-Venermo,M., Ranki,A. and Hedman,K. Submitting Institution Dept. of Virology, Univ. of Helsinki, POB 21 (Haartmaninkatu 3), Helsinki 00014, Finland
Original Host Homo sapiens Sample Type Skin and serum samples
Standardized Host Name Homo sapiens Host Tax Rank species
Standardized Host Class Mammalia Standardized Host Order Primates
Environmental Origin - N/A - Reagent Origin - N/A -
Number Reagent/Consumables Purpose PMID/Url Reference
1 Proteinase K Digestion of tissue samples for DNA purification. 12441066 View Related Publication
2 Phenol/chloroform Extraction of DNA from tissue samples.
3 Ethanol DNA precipitation during purification.
4 Nested PCR primers (for VP1, NS1, and VP2 genes) Amplification of specific genomic regions of the B19 virus for initial screening and further analysis.
5 Digoxigenin-labeled probes Detection of PCR products by hybridization.
6 TA-cloning system (Novagen Inc.) Cloning of PCR products for sequencing.
7 NheI and XhoI restriction enzymes Insertion of DNA sequences into plasmids for luciferase gene expression assay.
8 pGL3-Enhancer vector (Promega Corp.) Expression of luciferase reporter gene under control of different B19 promoter regions.
9 Firefly luciferase gene Reporter gene used to measure promoter activity.
10 UT7/Epo-S1 cells Cell line used for transfection and analysis of promoter activity.
11 Iscove's modified Dulbecco's medium (IMDM) Growth medium for UT7/Epo-S1 cells.
12 Erythropoietin Supplements UT7/Epo-S1 cell culture medium to support cell growth.
13 Cell culture lysis reagent Lysis of transfected cells to release luciferase for the assay.
14 Luciferase assay reagent Reactant for measuring luciferase activity.