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1
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Proteinase K
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Digestion of tissue samples for DNA purification.
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12441066
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2
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Phenol/chloroform
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Extraction of DNA from tissue samples.
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3
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Ethanol
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DNA precipitation during purification.
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4
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Nested PCR primers (for VP1, NS1, and VP2 genes)
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Amplification of specific genomic regions of the B19 virus for initial screening and further analysis.
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5
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Digoxigenin-labeled probes
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Detection of PCR products by hybridization.
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6
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TA-cloning system (Novagen Inc.)
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Cloning of PCR products for sequencing.
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7
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NheI and XhoI restriction enzymes
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Insertion of DNA sequences into plasmids for luciferase gene expression assay.
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8
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pGL3-Enhancer vector (Promega Corp.)
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Expression of luciferase reporter gene under control of different B19 promoter regions.
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9
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Firefly luciferase gene
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Reporter gene used to measure promoter activity.
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10
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UT7/Epo-S1 cells
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Cell line used for transfection and analysis of promoter activity.
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11
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Iscove's modified Dulbecco's medium (IMDM)
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Growth medium for UT7/Epo-S1 cells.
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12
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Erythropoietin
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Supplements UT7/Epo-S1 cell culture medium to support cell growth.
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13
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Cell culture lysis reagent
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Lysis of transfected cells to release luciferase for the assay.
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14
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Luciferase assay reagent
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Reactant for measuring luciferase activity.
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