Panoramic Parvovirus Strain Discovery Data Chain

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ParvoDB ID PV008687 GenBank Accession AF161225
Subfamily Parvovirinae Genus Erythroparvovirus
Species Erythroparvovirus primate1 Sequence Length 4268
Definition Erythrovirus B19 isolate Kati3 NS1 and VP1/2 genes, partial cds. Submission Date 1999-06-22
Strain Name Kati3 Sampling Date
Sampling Country None Location None
Submitted By Hokynar,K., Brunstein,J.D. and Hedman,K. Submitting Institution Dept. of Virology, Haartman Institute, University of Helsinki, Haartmaninkatu 3, Helsinki 00014, Finland
Original Host Homo sapiens Sample Type Serum and tissue samples
Standardized Host Name Homo sapiens Host Tax Rank species
Standardized Host Class Mammalia Standardized Host Order Primates
Environmental Origin - N/A - Reagent Origin - N/A -
Number Reagent/Consumables Purpose PMID/Url Reference
1 Basket forceps To obtain samples of synovial tissue during arthroscopy from patients. 10725428 View Related Publication
2 Synovial shaver To obtain samples of synovial tissue during arthroscopy from patients.
3 Commercial enzyme immunoassay (EIA) (Dako) To measure anti-B19 IgG antibodies in serum.
4 EIA of Biotrin To measure anti-B19 IgM antibodies in serum.
5 Proteinase K For DNA purification through digestion.
6 Phenol For DNA purification through extraction.
7 Ethanol For DNA precipitation during purification.
8 Glycogen Used as a carrier during DNA purification.
9 Viraemic serum containing 10^12 B19 genomes per ml Included as a positive control at a 10^-6 dilution in all PCR assays.
10 Water Used as a negative control in PCR assays.
11 Aerosol-resistant pipette tips and disposable racks Used to avoid carry-over and prevent false-positive results in PCR assays.
12 Perkin Elmer AmpliTaq Gold Used in PCR reactions.
13 Perkin-Elmer PCR buffer Used as the reaction buffer in PCR assays.
14 Ethidium bromide Used to stain PCR products for detection.
15 Nylon membrane Used for transfer and hybridization detection of PCR products.
16 Digoxigenin-labelled probe Used for hybridization detection of PCR products.
17 High Pure PCR product purification kit (Boehringer Mannheim) To purify PCR products before sequencing.
18 TAE–agarose gel Used for electrophoretic separation of PCR amplicons.
19 PVDF membrane For Southern blotting detection of PCR products.