Panoramic Parvovirus Strain Discovery Data Chain

Download Strain's Sequence
Feedback for This Record
ParvoDB ID PV008636 GenBank Accession OP019748
Subfamily Parvovirinae Genus Dependoparvovirus
Species Dependoparvovirus primate1 Sequence Length 4608
Definition UNVERIFIED: Adeno-associated virus 2 isolate CVR_8 sequence. Submission Date 2022-07-18
Strain Name CVR_8 Sampling Date 2022-04
Sampling Country United Kingdom Location Scotland, United Kingdom
Submitted By Ho,A., Orton,R., Tayler,R., Asamaphan,P., Tong,L., Smollett,K., Davis,C., Manali,M., McDonald,S.E., Pollock,L., Evans,C., McMenamin,J., Roy,K., Marsh,K., Divala,T., Holden,M., Lockhart,M., Yirrell,D., Currie,S., Shepherd,S.J., Jackson,C., Gunson,R., MacLean,A., McInnes,N., Battle,R., Hollenbach,J., Henderson,P., Chand,M., Hamilton,M.S., Estrada-Rivadeneyra,D., Levin,M., Robertson,D.L., Filipe,A., Willett,B., Breuer,J., Semple,M.G. VI, Turner,D., Baillie,J.K. and Thomson,E.C. Submitting Institution MRC-University of Glasgow Centre for Virus Research, University of Glasgow, Sir Michael Stoker Building, Glasgow, Scotland G61 1QH, UK
Original Host Homo sapiens; age = <24 Sample Type clinical samples
Standardized Host Name Homo sapiens Host Tax Rank species
Standardized Host Class Mammalia Standardized Host Order Primates
Environmental Origin - N/A - Reagent Origin - N/A -
Number Reagent/Consumables Purpose PMID/Url Reference
1 AAV2 pAAV-CAG-tdTomato viral preparation Used to coat plates for Anti-AAV2 ELISA Assay. 36996873 View Related Publication
2 Forward ITR primer Used for AAV2 qRT-PCR to detect a 62bp amplicon of the AAV2 inverted terminal repeat region.
3 Reverse ITR primer Used for AAV2 qRT-PCR to detect a 62bp amplicon of the AAV2 inverted terminal repeat region.
4 AAV2 ITR hydrolysis probe Used for AAV2 qRT-PCR to detect a 62bp amplicon of the AAV2 inverted terminal repeat region.
5 DNaseI (Ambion) Used to treat nucleic acid samples for improved detection of RNA viruses in metagenomic sequencing.
6 Ribo-Zero Plus rRNA Depletion Kit (Illumina) Used for ribosomal depletion in RNA virus detection for metagenomic sequencing.
7 SuperScript III (Invitrogen) Used for reverse transcription in RNA virus detection for metagenomic sequencing.
8 NEBNext Ultra II Non-Directional RNA Second Strand Synthesis Module (NEB) Used for double-strand DNA synthesis in RNA virus detection for metagenomic sequencing.
9 NEBNext Microbiome DNA Enrichment Kit (NEB) Used to partially remove host DNA for improved detection of DNA viruses in metagenomic sequencing.
10 KAPA LTP kit (Roche) Used for library preparation in metagenomic sequencing.
11 NEBNext Multiplex oligos for Illumina (NEB) Used for indexing in the library preparation for metagenomic sequencing.
12 VirCapSeq-VERT Capture Panel probes (Roche) Used for target enrichment sequencing, covering genomes of 207 viral taxa known to infect vertebrates.
13 Alkaline Phosphatase yellow (Merck) Used in Anti-AAV2 ELISA Assay for measuring absorbance.
14 Fixative solution (PFA:storage buffer) Used for post-staining fixation in spatial analysis (Codex Phenocycler).
15 PBS-T containing 5% BSA Used to block plates before sample addition in Anti-AAV2 ELISA Assay.
16 Anti-Human IgM (Merck) Used as secondary antibody in Anti-AAV2 ELISA Assay to detect human IgM.
17 Anti-Human IgG (Merck) Used as secondary antibody in Anti-AAV2 ELISA Assay to detect human IgG.
18 Goat Anti-Mouse IgG (Merck) Used as secondary antibody for the positive control in Anti-AAV2 ELISA Assay.
19 Custom conjugated antibodies (Akoya Biosciences) Used for staining in CO-Detection by indEXing (CODEX) analysis.
20 Target Specific RNA probes (Advanced Cell Diagnostics) Used for in situ hybridisation to detect AAV2, HHV6, and Adenovirus 41 RNA.
21 Vectamount mounting media (Vector Laboratories) Used for mounting stained slides for in situ hybridisation.