Panoramic Parvovirus Strain Discovery Data Chain
| ParvoDB ID | PV008607 | GenBank Accession | AM849099 |
| Subfamily | Parvovirinae | Genus | Bocaparvovirus |
| Species | Bocaparvovirus primate1 | Sequence Length | 417 |
| Definition | Human bocavirus partial VP2 gene for capsid protein, isolate Isr127444. | Submission Date | 2007-08-21 |
| Strain Name | Isr127444 | Sampling Date | from 2016-01 to 2016-12 |
| Sampling Country | Israel | Location | Israel |
| Submitted By | Hindiyeh,M.Y., Keller,N., Mandelboim,M., Rubinov,J., Regev,L., Ram,D., Levy,V., Orzitzer,S., Shaharabani,H., Azar,R., Mendelson,E. and Grossman,Z. | Submitting Institution | Hindiyeh M.Y., Israel Central Virology Laboratory, Chaim Sheba Medical Center, Tel-Hashomer, 52621, ISRAEL |
| Original Host | Homo sapiens | Sample Type | throat swab patient samples |
| Standardized Host Name | Homo sapiens | Host Tax Rank | species |
| Standardized Host Class | Mammalia | Standardized Host Order | Primates |
| Environmental Origin | - N/A - | Reagent Origin | - N/A - |
| Number | Reagent/Consumables | Purpose | PMID/Url | Reference |
|---|---|---|---|---|
| 1 | QIAamp DNA blood Mini kit (Qiagen GmbH, Hilden, Germany) | Extraction of viral genomic DNA from patient samples. | 17977996 | View Related Publication |
| 2 | High Pure viral RNA extraction kit (Roche Diagnostics GmbH, Mannheim, Germany) | Extraction of viral genomic RNA from patient samples. | ||
| 3 | TaqMan chemistry | Amplification of adenovirus, HBoV, influenza A and B, and RSV A and B by real-time PCR. | ||
| 4 | Gel detection | Testing for parainfluenza viruses 1, 2, and 3 by reverse transcription-PCR. | ||
| 5 | BocaSEQ3 (forward) and BocaSEQ4 (reverse) primers | Phylogenetic analysis of the VP2 genes of HBoV-positive samples. | ||
| 6 | Qiagen QIAquick gel extraction | Purification of PCR products. | ||
| 7 | ABI Prism dye Deoxy Terminator cycle sequencing kit (Applied Biosystems, Foster City, CA) | Sequencing the purified PCR products. |