Panoramic Parvovirus Strain Discovery Data Chain
| ParvoDB ID | PV008558 | GenBank Accession | Z70598 |
| Subfamily | Parvovirinae | Genus | Erythroparvovirus |
| Species | Erythroparvovirus primate1 | Sequence Length | 446 |
| Definition | Parvovirus B19 DNA, patient S752, genome position 3982-4428. | Submission Date | 1996-04-03 |
| Strain Name | patient S752 | Sampling Date | from 1991 to 1994 |
| Sampling Country | None | Location | Institut für Medizinische Mikrobiologie und Hygiene, Universität Regensburg, Germany; Institut für Virologie, Universität Wien, Austria; Max von Pettenkofer-Institut, München, Germany; Rheumaklinik Olsberg, Germany; Hôpital Cantonal Universitaire de Geneve, Division de Rheumatologie, Genève, Switzerland; Department of Virology, University of Helsinki, Helsinki, Finland, |
| Submitted By | Hemauer,A., Von Poblotzki,A., Gigler,A., Cassinotti,P., Siegl,G., Wolf,H. and Modrow,S. | Submitting Institution | A. Hemauer, Inst. f. Med. Mikrobiologie, und Hygiene, Franz-Josef- Strauss Allee 11, D- 93053 Regensburg, FRG |
| Original Host | Homo sapiens | Sample Type | serum samples |
| Standardized Host Name | Homo sapiens | Host Tax Rank | species |
| Standardized Host Class | Mammalia | Standardized Host Order | Primates |
| Environmental Origin | - N/A - | Reagent Origin | - N/A - |
| Number | Reagent/Consumables | Purpose | PMID/Url | Reference |
|---|---|---|---|---|
| 1 | Water | Dilution of serum samples for PCR preparation. | 8760426 | View Related Publication |
| 2 | dNTPs (Perkin Elmer) | Used in PCR for nucleotides. | ||
| 3 | Primers | Used for nested PCR reactions. | ||
| 4 | PCR buffer for Pwo polymerase | Provides a suitable environment for the polymerase enzyme to function during PCR. | ||
| 5 | Pwo DNA polymerase (Boehringer Mannheim) | Polymerase enzyme with proof-reading activity used for PCR amplification. | ||
| 6 | Ethidium bromide | Staining DNA fragments after electrophoresis on an agarose gel. | ||
| 7 | Agarose gel | Used in electrophoresis to separate DNA fragments. | ||
| 8 | Qiagen PCR purification kit | Purification of PCR fragments before sequencing. |