Panoramic Parvovirus Strain Discovery Data Chain

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ParvoDB ID PV008549 GenBank Accession Z70589
Subfamily Parvovirinae Genus Erythroparvovirus
Species Erythroparvovirus primate1 Sequence Length 423
Definition Parvovirus B19 DNA, patient S471, genome position 2978-3401. Submission Date 1996-04-03
Strain Name patient S471 Sampling Date from 1991 to 1994
Sampling Country None Location Institut für Medizinische Mikrobiologie und Hygiene, Universität Regensburg, Germany; Institut für Virologie, Universität Wien, Austria; Max von Pettenkofer-Institut, München, Germany; Rheumaklinik Olsberg, Germany; Hôpital Cantonal Universitaire de Geneve, Division de Rheumatologie, Genève, Switzerland; Department of Virology, University of Helsinki, Helsinki, Finland,
Submitted By Hemauer,A., Von Poblotzki,A., Gigler,A., Cassinotti,P., Siegl,G., Wolf,H. and Modrow,S. Submitting Institution A. Hemauer, Inst. f. Med. Mikrobiologie, und Hygiene, Franz-Josef- Strauss Allee 11, D- 93053 Regensburg, FRG
Original Host Homo sapiens Sample Type serum samples
Standardized Host Name Homo sapiens Host Tax Rank species
Standardized Host Class Mammalia Standardized Host Order Primates
Environmental Origin - N/A - Reagent Origin - N/A -
Number Reagent/Consumables Purpose PMID/Url Reference
1 Water Dilution of serum samples for PCR preparation. 8760426 View Related Publication
2 dNTPs (Perkin Elmer) Used in PCR for nucleotides.
3 Primers Used for nested PCR reactions.
4 PCR buffer for Pwo polymerase Provides a suitable environment for the polymerase enzyme to function during PCR.
5 Pwo DNA polymerase (Boehringer Mannheim) Polymerase enzyme with proof-reading activity used for PCR amplification.
6 Ethidium bromide Staining DNA fragments after electrophoresis on an agarose gel.
7 Agarose gel Used in electrophoresis to separate DNA fragments.
8 Qiagen PCR purification kit Purification of PCR fragments before sequencing.