Panoramic Parvovirus Strain Discovery Data Chain
| ParvoDB ID | PV008424 | GenBank Accession | JN794565 |
| Subfamily | Parvovirinae | Genus | Bocaparvovirus |
| Species | Bocaparvovirus primate1 | Sequence Length | 5299 |
| Definition | Human bocavirus isolate GZ4785, complete genome. | Submission Date | 2011-09-28 |
| Strain Name | GZ4785 | Sampling Date | 2010-08-28 |
| Sampling Country | China | Location | Guangzhou, China |
| Submitted By | He,X., Li,M.F., Xu,L., Guan,L.L., Zheng,Y., Zhang,D.M., Feng,F.S., Wang,Z., Deng,Y., Ling,M.J., Luo,Y.F., Wang,Y., Wu,J.H., Yuan,G.Q. and Cao,K.Y. | Submitting Institution | Ministry of Education Key Laboratory of Tropical Disease Control, Sun Yat-sen University, Zhongshan School of Medicine, 74 Zhongshan 2nd Road, Guangzhou, Guangdong 510080, China |
| Original Host | Homo sapiens | Sample Type | throat swabs |
| Standardized Host Name | Homo sapiens | Host Tax Rank | species |
| Standardized Host Class | Mammalia | Standardized Host Order | Primates |
| Environmental Origin | - N/A - | Reagent Origin | - N/A - |
| Number | Reagent/Consumables | Purpose | PMID/Url | Reference |
|---|---|---|---|---|
| 1 | Earle's Balanced Salt Solution (BioSource International, USA) | Part of the Viral Transport Medium (VTM) used for throat swab specimens. | 22984581 | View Related Publication |
| 2 | Bovine serum albumin | Part of the Viral Transport Medium (VTM) to stabilize the virus in throat swab specimens. | ||
| 3 | Vancomycin | An antibiotic included in VTM to prevent bacterial contamination. | ||
| 4 | Amikacin | An antibiotic included in VTM to prevent bacterial contamination. | ||
| 5 | Nystatin | An antifungal included in VTM to prevent fungal contamination. | ||
| 6 | QIAamp MiniElute Virus Spin (QIAGEN, Germany) | Kit used for simultaneous extraction of DNA and RNA from throat swab specimens. | ||
| 7 | Superscript III transcriptase (Invitrogen, Life Technology, USA) | Used for reverse transcription of virus RNA. | ||
| 8 | Random hexamer primers (Invitrogen, Life Technology, USA) | Used for reverse transcription of virus RNA. | ||
| 9 | Primers and probes (synthesized by Invitrogen, Life Technology, USA) | Used in real-time PCR for detecting HBoV by targeting the NP1 region. | ||
| 10 | iQ Supermix reaction mixture (Bio-Rad, USA) | Component of the reaction mixture in real-time PCR. | ||
| 11 | Platinum pfx Taq polymerase (Invitrogen, USA) | Used in PCR to amplify the complete genome of HBoV strains. | ||
| 12 | Agarose gel DNA purification kit (Takara, China) | Used for purifying PCR products for genome analysis. | ||
| 13 | Zero Blunt TOPO PCR cloning kit for sequencing (Invitrogen, USA) | Used for cloning the terminal sequences of HBoV genomes for sequencing. |