ParvoDB ID PV008380 GenBank Accession OQ708493
Subfamily Parvovirinae Genus Protoparvovirus
Species Protoparvovirus incertum1 Sequence Length 4424
Definition Tusavirus 1 strain GZ814 NS1, VP1, and VP2 genes, complete cds. Submission Date 2023-03-28
Strain Name GZ814 Sampling Date from 2018-02 to 2022-07
Sampling Country China Location Guangzhou, China
Submitted By He,H. Submitting Institution School of Public Health, Southern Medical University, Guangzhou North Road, Guangzhou, Guangdong 510515, China
Original Host Homo sapiens Sample Type Faecal samples
Standardized Host Name Homo sapiens Host Tax Rank species
Standardized Host Class Mammalia Standardized Host Order Primates
Environmental Origin - N/A - Reagent Origin - N/A -
Number Reagent/Consumables Purpose PMID/Url Reference
1 Phosphate-buffered saline Used to store collected feces samples. 37396384 View Related Publication
2 MiniBEST Viral RNA/DNA Extraction Kit (TaKaRa Biotechnology, Kusatsu, Japan) Used for extracting DNA from the fecal sample supernatants.
3 Green Master Mix (Promega, Fitchburg, WI, USA) Used in the PCR mixture.
4 Primers Used for nested PCR targeting the partial NS1 region to detect the presence of TuV DNA.
5 Agarose gels (1.0%) Used for analyzing PCR products through electrophoresis.
6 Sterilized H2O Used in the PCR mixture.