Panoramic Parvovirus Strain Discovery Data Chain

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ParvoDB ID PV007903 GenBank Accession LC760833
Subfamily Parvovirinae Genus Protoparvovirus
Species Protoparvovirus primate3 Sequence Length 4455
Definition Cutavirus JPN-SKC138H genes for NS1, VP1, VP2, partial and complete cds. Submission Date 2023-03-20
Strain Name JPN-SKC138H Sampling Date 2015
Sampling Country Japan Location Japan
Submitted By Hashida,Y. and Daibata,M. Submitting Institution Contact:Yumiko Hashida Kochi medical school, Kochi university, Department of microbiology and infection; Kohasu Oko-cho, Nankoku, Kochi 783-8505, Japan
Original Host Homo sapiens Sample Type skin swab samples
Standardized Host Name Homo sapiens Host Tax Rank species
Standardized Host Class Mammalia Standardized Host Order Primates
Environmental Origin - N/A - Reagent Origin - N/A -
Number Reagent/Consumables Purpose PMID/Url Reference
1 Sterile cotton swabs moistened with phosphate-buffered saline (PBS) Sample collection by rubbing the skin. 37069566 View Related Publication
2 Phosphate-buffered saline (PBS) Moistening the sterile cotton swabs for sample collection.
3 TaqMan Gene Expression Master Mix Preparation of the reaction mixture for real-time quantitative PCR (qPCR).
4 Primers for TaqMan-based real-time quantitative PCR (qPCR) targeting CuV VP2 region Amplification of CuV DNA for detection and quantification.
5 Dual-labeled probe for qPCR Binding to the specific region of CuV DNA for detection in qPCR.
6 Water Negative control in the qPCR reaction mixture.
7 RNase P gene primers Positive control to confirm the presence of PCR-amplifiable DNA.
8 pMD20-T vector (Takara Bio) Cloning the PCR product for generating a standard curve to calculate viral copy number.
9 Phenol: chloroform DNA extraction.
10 BioPhotometer Plus (Eppendorf) Measurement of extracted DNA concentrations.