Panoramic Parvovirus Strain Discovery Data Chain

Download Strain's Sequence
Feedback for This Record
ParvoDB ID PV007876 GenBank Accession LC744019
Subfamily Parvovirinae Genus Protoparvovirus
Species Protoparvovirus primate3 Sequence Length 4455
Definition Cutavirus JPN-SKCA36H genes for NS1, VP1, VP2, partial and complete cds. Submission Date 2022-12-19
Strain Name JPN-SKCA36H Sampling Date 2019
Sampling Country Japan Location Japan
Submitted By Hashida,Y. and Daibata,M. Submitting Institution Contact:Yumiko Hashida Kochi medical school, Kochi university, Department of microbiology and infection; Kohasu Oko-cho, Nankoku, Kochi 783-8505, Japan
Original Host Homo sapiens Sample Type skin swab samples
Standardized Host Name Homo sapiens Host Tax Rank species
Standardized Host Class Mammalia Standardized Host Order Primates
Environmental Origin - N/A - Reagent Origin - N/A -
Number Reagent/Consumables Purpose PMID/Url Reference
1 Sterile cotton swabs moistened with phosphate-buffered saline (PBS) Sample collection by rubbing the skin. 37069566 View Related Publication
2 Phosphate-buffered saline (PBS) Moistening the sterile cotton swabs for sample collection.
3 TaqMan Gene Expression Master Mix Preparation of the reaction mixture for real-time quantitative PCR (qPCR).
4 Primers for TaqMan-based real-time quantitative PCR (qPCR) targeting CuV VP2 region Amplification of CuV DNA for detection and quantification.
5 Dual-labeled probe for qPCR Binding to the specific region of CuV DNA for detection in qPCR.
6 Water Negative control in the qPCR reaction mixture.
7 RNase P gene primers Positive control to confirm the presence of PCR-amplifiable DNA.
8 pMD20-T vector (Takara Bio) Cloning the PCR product for generating a standard curve to calculate viral copy number.
9 Phenol: chloroform DNA extraction.
10 BioPhotometer Plus (Eppendorf) Measurement of extracted DNA concentrations.