ParvoDB ID PV007870 GenBank Accession MH910506
Subfamily Parvovirinae Genus Bocaparvovirus
Species Bocaparvovirus primate1 Sequence Length 760
Definition Human bocavirus isolate Iraq3 viral capsid protein (vp1/vp2) gene, partial cds. Submission Date 2018-09-10
Strain Name Iraq3 Sampling Date from 2017 to 2018
Sampling Country Iraq Location Iraq
Submitted By Hasan,D.A., Hussein,A.A., Al-Mayah,Q.S. and Aufi,I.M. Submitting Institution Medical microbiology, Diyala university college of medicine, Al Baladiyat, Baghdad, Baghdad 10061, Iraq
Original Host Homo sapiens Sample Type nasophryngeal swab
Standardized Host Name Homo sapiens Host Tax Rank species
Standardized Host Class Mammalia Standardized Host Order Primates
Environmental Origin - N/A - Reagent Origin - N/A -
Number Reagent/Consumables Purpose PMID/Url Reference
1 Sigma Virocult Swabs Collection of nasopharyngeal samples with viral transport medium https://www.researchgate.net/publication/339982318_Phylogenetic_Analysis_of_Human_Bocavirus_Isolated_from_Children_with_Lower_Respiratory_Tract_Infections_in_Baghdad_Iraq View Related Publication
2 Commercial Kit (Instant virus DNA/RNA, Cat. No. 845-KS-4500250, German) DNA extraction from nasopharyngeal swab specimens
3 Primers (VP-A, VP-B, VP-C) Amplification of fragments of human bocavirus VP1/2 capsid gene
4 TBE buffer Preparation of a stock solution of primers
5 Distilled water Preparation of working solution and reaction mixture in PCR
6 Master Mix Facilitate PCR amplification as part of the mixture
7 Ethidium bromide Staining Gel electrophoresis products for visualization under UV light