Panoramic Parvovirus Strain Discovery Data Chain
| ParvoDB ID | PV007684 | GenBank Accession | JX434056 |
| Subfamily | Parvovirinae | Genus | Bocaparvovirus |
| Species | Bocaparvovirus primate1 | Sequence Length | 1017 |
| Definition | Human bocavirus isolate CQ279 VP1/VP2 gene, partial cds. | Submission Date | 2012-07-27 |
| Strain Name | CQ279 | Sampling Date | 2009-12 |
| Sampling Country | China | Location | the Children’s Hospital of Chongqing Medical University, China |
| Submitted By | Hao,R. and Liu,E.M. | Submitting Institution | Department of Respiratory Medicine, Children's Hospital of Chongqing Medical University, Zhong Shan Er Road No. 136, Yu Zhong District, Chongqing 400014, China |
| Original Host | Homo sapiens | Sample Type | tract samples |
| Standardized Host Name | Homo sapiens | Host Tax Rank | species |
| Standardized Host Class | Mammalia | Standardized Host Order | Primates |
| Environmental Origin | - N/A - | Reagent Origin | - N/A - |
| Number | Reagent/Consumables | Purpose | PMID/Url | Reference |
|---|---|---|---|---|
| 1 | QIAamp MinElute Virus Spin kit (Qiagen) | For viral DNA and RNA extraction from NPA samples. | 23303830 | View Related Publication |
| 2 | SuperScriptII First-Strand Synthesis System (Invitrogen) | For cDNA synthesis from RNA. | ||
| 3 | TaqMan Universal Master Mix (Applied Biosystems) | Used in the real-time PCR reaction mixture. | ||
| 4 | HBoV1F primer | Forward primer for real-time PCR targeting HBoV1 NS1 gene. | ||
| 5 | HBoV1R primer | Reverse primer for real-time PCR targeting HBoV1 NS1 gene. | ||
| 6 | HBoV1 probe (FAM)-59-CCAGAGATGTTCACTCGCCG-39-MGB - BHQ1 | Probe for real-time PCR targeting HBoV1 NS1 gene. | ||
| 7 | pMD19-T vector (Takara) | For cloning the amplified target fragment from real-time PCR. | ||
| 8 | Primers for amplification of HBoV1 full and partial sequences | For sequencing PCR products from HBoV1-positive cases. |