ParvoDB ID PV007648 GenBank Accession GQ281565
Subfamily Parvovirinae Genus Bocaparvovirus
Species Bocaparvovirus primate2 Sequence Length 483
Definition Human bocavirus 2 isolate ST411 nonstructural protein NS1 gene, partial cds. Submission Date 2009-06-17
Strain Name ST411 Sampling Date 2009-01-12
Sampling Country South Korea Location Sanggyepaik Hospital, Seoul,Korea, South Korea
Submitted By Han,T.-H., Chung,J.-Y., Park,S.-H. and Hwang,E.-S. Submitting Institution Department of Laboratory Medicine and Pediatrics, Sanggyepaik Hospital, Inje University College of Medicine, 761-1 Sanggye-7-Dong, Nowon-Gu, Seoul 139-707, Republic of Korea
Original Host Homo sapiens Sample Type stool samples
Standardized Host Name Homo sapiens Host Tax Rank species
Standardized Host Class Mammalia Standardized Host Order Primates
Environmental Origin - N/A - Reagent Origin - N/A -
Number Reagent/Consumables Purpose PMID/Url Reference
1 Phosphate-buffered saline Dilution of stool samples for preparation. 19862470 View Related Publication
2 Rotaclone ELISA kit Identification of rotavirus in stool samples.
3 Adenoclone 40/41 ELISA kit Identification of adenovirus 40 and 41 in stool samples.
4 Tri-reagent Extraction of total RNA from stool suspensions.
5 DEPC-treated distilled water Dissolving final RNA pellets after extraction.
6 QIAamp DNA stool Kit DNA extraction from stool samples.
7 Primers (SLV5317, SLV5749) Detection of sapovirus via PCR.
8 Primers (Mon 340, Mon 348) RT-PCR detection of human astrovirus (HAstV).
9 QIAquick Amplicon purification before sequencing.
10 BigDye Terminator v3.1 Cycle Sequencing kit Sequencing of PCR products.