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1
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MgCl2
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To prepare samples by adding 1 M MgCl2 to reach a final concentration of 0.05 M for each 1 l of samples.
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28233174
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2
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HA nitrocellulose membrane, 0.45 μm pore size, 142 mm diameter
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Used in the filtration process to concentrate viruses by the virus adsorption elution method.
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3
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0.5 mM H2SO4, pH 4
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To rinse the membrane prior to virus recovery.
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4
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Organic elution buffer (3% beef extract, 0.05 M glycine pH 9.4)
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Used to elute viruses from the membrane.
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5
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QIAamp DNA Blood Mini Kit
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For viral nucleic acid extraction from concentrated suspension.
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6
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Murine norovirus
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Used as an internal control to identify the occurrence of PCR inhibition.
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7
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Nuclease free water
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Used as a negative control in qPCR assays.
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8
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Quantitect probe PCR kit with uracil-N-glycosylase (UNG)
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Used in real-time qPCR reactions.
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9
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SYBR green
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Used in SYBR green qPCR assay for HBoV-2, 3, and 4 quantification.
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10
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StrataClone PCR cloning kit
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Used for cloning PCR fragments into the pSC-Aamp/kan vector for standard preparation.
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11
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QIAquick PCR Purification Kit
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Used to purify PCR products for standard preparation and sequencing.
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12
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Quant-iT dsDNA HS Assay (Invitrogen)
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Used to determine the nucleic acid concentrations of the purified products.
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13
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Avogadro’s constant
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Referenced for determining the number of DNA copies.
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14
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Rotorgene 3000 cycler system
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Used for the amplification and data analysis of real-time qPCR reactions.
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