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1
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Primary human liver samples
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Source of naturally hepatotropic AAV variants
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32908003
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View Related Publication
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2
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HuH-7 cells
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Cell line used for testing AAV transduction and replication
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3
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Human adenovirus 5 (hAd5)
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Co-infection with RC-AAV to support AAV replication
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4
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Fah//Rag2//Il2rg/ (FRG) mice with humanized livers
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In vivo model for testing AAV tropism and transduction efficiency
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5
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ssAAV-LSP1-GFP-BC-WPRE-BGHpA
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Barcoded vector used for transduction efficiency assays
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6
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Heparin
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Used in competition assays to infer HSPG-binding affinity
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7
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Immunohistochemical reagents (antibodies for human GAPDH, GFP, and DAPI)
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Visualization and quantification of hepatocyte transduction in liver sections
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8
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QIAamp Fast DNA Tissue Kit
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DNA extraction from human liver tissues
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9
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Multiplex polymerase chain reaction (PCR) primers
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Amplification of AAV capsid and rep genes from isolated DNA
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10
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SYBR Green qPCR
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Quantification of AAV genomes during replication studies
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11
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Isotonic Percoll
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Separation of live and dead cells during single-cell suspension preparation
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12
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Phycoerythrin-conjugated anti-human-HLA-ABC, biotin-conjugated anti-mouse-H2Kb, allophycocyanin-conjugated streptavidin
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Flow cytometry reagents for differentiating human and mouse hepatocytes
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13
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Iodixanol
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AAV purification via gradient ultracentrifugation
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14
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HiTrap Heparin HP column
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Heparin affinity chromatography to evaluate AAV capsid interaction
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