Human bocavirus 2 isolate 271-BJ07 capsid protein (vp1) gene, partial cds.
Submission Date
2010-08-16
Strain Name
271-BJ07
Sampling Date
from 2006-03 to 2007-11
Sampling Country
China
Location
Beijing, China, China
Submitted By
Guo,L. and Wang,J.
Submitting Institution
State Key Laboratory for Molecular Virology and Genetic Engineering, Institute of Pathogen Biology, 9# Dong Dan San Tiao, Dongcheng District, Beijing 100730, China
Nested polymerase chain reaction (PCR) using primers targeting the VP1/2 region
Amplification of HBoVs and the subsequent detection of a 576-bp fragment.
3
pMD18-T vector (Takara, Dalian, China)
Cloning PCR products for HBoV species determination by sequencing.
4
Enzyme-linked immunosorbent assay (ELISA)
Identification of co-infections with other AGE viruses such as rotavirus, norovirus, sapovirus, astrovirus, and adenovirus.
5
Reverse transcriptase PCR (RT-PCR)
Identification of co-infections with other AGE viruses such as rotavirus, norovirus, sapovirus, astrovirus, and adenovirus.
6
Specific overlapping primer sets based on published HBoV3 sequences
Amplification of a nearly full-length genome sequence of the HBoV3 strain 46-BJ07.
7
Modified protocol for RACE (Invitrogen, Carlsbad, CA)
Amplification of the 5′- and 3′- terminal sequences.
8
SimPlot software
Generation of similarity plots for the HBoV genomes (Note: software, listed for completeness but noted as excluded from the list).
9
MEGA 4.0 software
Multiple sequence alignments and generation of neighbor-joining trees with 1,000 bootstrap replicates (Note: software, listed for completeness but noted as excluded from the list).
