Panoramic Parvovirus Strain Discovery Data Chain
| ParvoDB ID | PV007052 | GenBank Accession | HQ871584 |
| Subfamily | Parvovirinae | Genus | Bocaparvovirus |
| Species | Bocaparvovirus primate1 | Sequence Length | 574 |
| Definition | Human bocavirus 1 isolate BCH1348 VP1 gene, partial cds. | Submission Date | 2011-01-10 |
| Strain Name | BCH1348 | Sampling Date | from 2008-03 to 2010-07 |
| Sampling Country | China | Location | Beijing, China, China |
| Submitted By | Guo,L. and Wang,J. | Submitting Institution | State Key Laboratory for Molecular Virology and Genetic Engineering, Institute of Pathogen Biology, 9# Dong Dan San Tiao, Dongcheng District, Beijing 100730, China |
| Original Host | Homo sapiens | Sample Type | Nasopharyngeal aspirates |
| Standardized Host Name | Homo sapiens | Host Tax Rank | species |
| Standardized Host Class | Mammalia | Standardized Host Order | Primates |
| Environmental Origin | - N/A - | Reagent Origin | - N/A - |
| Number | Reagent/Consumables | Purpose | PMID/Url | Reference |
|---|---|---|---|---|
| 1 | Nasopharyngeal aspirates | Collected samples from children with acute lower respiratory tract infections. | 21888826 | View Related Publication |
| 2 | NucliSens easyMAG system (bioMérieux, Marcy l’Etoile, France) | Extracted viral nucleic acid from nasopharyngeal aspirates. | ||
| 3 | Primers targeting the viral proteins (VP) 1/2 region | Used in nested PCR with touch-down procedure to screen for HBoV1–4. | ||
| 4 | PCR master mixture | Preparation used for PCR process. | ||
| 5 | Controls for nucleic acid extraction and PCR analyses | Monitored contamination during the process. | ||
| 6 | GenBank (accession nos. HQ871520–HQ871650 and HQ871664–HQ871673) | Reference for sequence alignment and phylogenetic analysis of PCR amplicons. | ||
| 7 | Reference sequences from GenBank (accession nos. FJ948861, NC_012564, EU918736, HM132056, and HQ152935) | Used for multiple-alignment analysis of sequences. |