Panoramic Parvovirus Strain Discovery Data Chain

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ParvoDB ID PV006985 GenBank Accession JQ240469
Subfamily Parvovirinae Genus Bocaparvovirus
Species Bocaparvovirus primate1 Sequence Length 1629
Definition Human bocavirus 1 isolate 111-BJ07 VP2 (vp2) gene, complete cds. Submission Date 2011-12-05
Strain Name 111-BJ07 Sampling Date 2007-11
Sampling Country China Location Beijing, China, China
Submitted By Guo,L. and Wang,J. Submitting Institution State Key Laboratory for Molecular Virology and Genetic Engineering, and Christophe Merieux Laboratory, IPB, CAMS-Fondation Merieux, Institute of Pathogen Biology (IPB), Chinese Academy of Medical Sciences (CAMS), 9 Dong Dan San Tiao, Dong Cheng District, Beijing 100730, China
Original Host Homo sapiens Sample Type serum
Standardized Host Name Homo sapiens Host Tax Rank species
Standardized Host Class Mammalia Standardized Host Order Primates
Environmental Origin - N/A - Reagent Origin - N/A -
Number Reagent/Consumables Purpose PMID/Url Reference
1 VP2 genes of HBoV1-4 To produce antigens for evaluating the seroprevalence of HBoVs. 22761854 View Related Publication
2 Baculovirus expression system For expression of VP2 genes in insect cells to generate VLPs.
3 Stool samples Source of VP2 genes of HBoV1-3 for amplification by PCR.
4 Electron microscopy (EM) To verify the morphology of generated HBoV VLPs.
5 Murine antisera To verify the reactivity of purified VLPs in Western blot assays.
6 HBoV VLPs Used as coating antigens in ELISA assays for detecting antibodies.
7 Negative sera samples from infants aged 0-6 months Used as control negative samples in Western blot analysis.
8 Human parvovirus B19 VP2 protein Test reactivity and serve as a control in ELISA assays.
9 Influenza virus H5 hemagglutinin antiserum Used as a control in ELISA assays to compare reactivity.
10 High Five cells Infected with recombinant baculovirus for VLP production.
11 CsCl solutions Used in density gradient centrifugation for VLP purification.
12 BSA (bovine serum albumin) Blocking agent in ELISA assays.