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1
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Guanidine buffer
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Used for nucleic acid extraction from nasopharyngeal aspirates (NPA) by the method of Boom et al.
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22358354
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2
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Silica
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Used in combination with guanidine buffer for nucleic acid extraction.
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3
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Lysis buffer
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Component of the nucleic acid extraction process (contains Tris pH 6.4, EDTA pH 8.0, Triton X-100, and guanidine isothiocyanate).
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4
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Ethanol (70% v/v)
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Used to wash silica-nucleic acid pellet during the extraction process.
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5
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Acetone
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Used to wash silica-nucleic acid pellet during the extraction process.
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6
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Elution buffer (TE buffer, pH 8.0, without RNase inhibitor)
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Used to elute DNA and RNA from the silica-nucleic acid pellet after the washing steps.
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7
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dNTPs
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Component of the PCR reaction for amplification of HBoV DNA.
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8
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Primers (forward and reverse)
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Used for the amplification of a specific region of HBoV DNA by PCR.
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9
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MgCl2
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Component of the PCR reaction mix to provide necessary ions for DNA polymerase activity.
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10
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Platinum Taq DNA polymerase (Invitrogen)
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Enzyme used in the PCR reaction to amplify HBoV DNA.
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11
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Polyacrylamide gels
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Used to visualize PCR products stained with silver solution.
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12
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Silver solution (0.11 M AgNO3)
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Used to stain polyacrylamide gels for visualization of PCR products.
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13
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EX Taq Hot Start Version DNA polymerase (TaKaRa)
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Enzyme used in nested PCR for the amplification of the NP1 protein region.
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14
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QIAquick gel extraction kit (Qiagen)
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Used for purification of PCR amplified fragments following agarose-gel electrophoresis.
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15
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Cycle-sequencing kits (ABI PRISM BigDye Terminator v. 3.1; PE Applied Biosystems)
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Used for sequencing reactions performed bidirectionally.
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