|
1
|
DEAE-dextran
|
To supplement Caco-2 cultures and enhance virus infection.
|
28777951
|
View Related Publication
|
|
2
|
Eagle's Minimum Essential Medium (EMEM) (GIBCO)
|
Culture medium for preparing the inoculum and maintaining cell cultures.
|
|
3
|
Penicillin-streptomycin
|
Antibiotic supplement in EMEM to prevent bacterial contamination.
|
|
4
|
Gentamicin
|
Alternative antibiotic supplement in EMEM to prevent bacterial contamination.
|
|
5
|
Glutamine
|
Supplement in EMEM to support cell growth.
|
|
6
|
Fetal bovine serum (FBS)
|
Supplement in EMEM for complete growth medium.
|
|
7
|
Trypsin and EDTA
|
To trypsinize and reseed cell monolayers.
|
|
8
|
Phosphate-buffered saline (PBS)
|
For washing cultures.
|
|
9
|
Axyprep Body Fluid Viral DNA/RNA Miniprep Kit (Axygen Bioscience)
|
Nucleic acid extraction from supernatant and monolayers.
|
|
10
|
Platinum Taq DNA polymerase (Invitrogen)
|
For qPCR and PCR reactions.
|
|
11
|
SYBR Green (Invitrogen S-7563)
|
Fluorescent dye for qPCR.
|
|
12
|
Silver solution (0.11 M AgNO3)
|
Staining PCR products in polyacrylamide gels.
|
|
13
|
Methanol
|
Fixing cell monolayers for staining.
|
|
14
|
Hematoxylin-eosin
|
Staining fixed cell monolayers.
|
|
15
|
Paraformaldehyde
|
Fixing cells for immunofluorescence assay (IFA).
|
|
16
|
NP40
|
Permeabilizing cells for IFA.
|
|
17
|
Bovine serum albumin
|
Blocking solution in IFA.
|
|
18
|
FITC-anti-human IgG
|
Secondary antibody for IFA.
|
|
19
|
FluorSave (EMD Millipore)
|
Mounting medium for IFA slides.
|
|
20
|
GelRed (Biotum)
|
Staining PCR products in agarose gels.
|
|
21
|
QIAGEN PCR cleanup kit
|
Purification of PCR products.
|
|
22
|
ABI PRISM BigDye Terminator v. 3.1 (PE Applied Biosystems)
|
Cycle-sequencing for DNA sequencing analysis.
|
