Panoramic Parvovirus Strain Discovery Data Chain

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ParvoDB ID PV006578 GenBank Accession KC878501
Subfamily Parvovirinae Genus Bocaparvovirus
Species Bocaparvovirus primate1 Sequence Length 660
Definition Human bocavirus 1 isolate 3192_HN_12 nonstructural protein 1 (NP1) gene, complete cds. Submission Date 2013-04-09
Strain Name 3192_HN_12 Sampling Date from 2011-01 to 2012-12
Sampling Country Argentina Location Argentina
Submitted By Ghietto,L.M. and Adamo,M.P. Submitting Institution Instituto de Virologia, Universidad Nacional de Cordoba, Enfermera Gordillo Gomez S/N, Cordoba, Cordoba 5016, Argentina
Original Host Homo sapiens Sample Type Respiratory specimens
Standardized Host Name Homo sapiens Host Tax Rank species
Standardized Host Class Mammalia Standardized Host Order Primates
Environmental Origin - N/A - Reagent Origin - N/A -
Number Reagent/Consumables Purpose PMID/Url Reference
1 guanidinium buffer and silica Nucleic acids extraction from 100 ll of NPA or NPS specimens. 25269520 View Related Publication
2 Platinum Taq DNA polymerase (Invitrogen) Used for amplification in real-time PCR (Applied Biosystems 7500 Real-Time PCR System) of the NP1 gene for relative quantification of HBoV1.
3 SYBR Green (Invitrogen S-7563) Added to the amplification reaction mixture for detection in real-time PCR.
4 AgPath-IDTM One-Step RT-PCR Reagents (Applied Biosystems) qRT-PCR for detection of other viral pathogens including Flu A and B viruses, PIV1, -2 and -3, AdV, RSV and MPV in HBoV-positive samples.
5 dual-labeled FAM-BHQ1 probes Used in PCR assay as part of the detection system for various respiratory pathogens.
6 QIAGEN PCR cleanup kit Purification of PCR products prior to sequencing.
7 ABI PRISM BigDye Terminator v. 3.1 (PE Applied Biosystems) Used for sequencing reactions of amplified NP1 and VP1 regions.
8 ethidium bromide Staining of agarose gels to resolve PCR products visually.