Panoramic Parvovirus Strain Discovery Data Chain
| ParvoDB ID | PV006562 | GenBank Accession | KP299161 |
| Subfamily | Parvovirinae | Genus | Erythroparvovirus |
| Species | Erythroparvovirus primate1 | Sequence Length | 863 |
| Definition | Human parvovirus B19 isolate 379 NS1 (NS1) and VP1u (VP1u) genes, partial cds. | Submission Date | 2014-12-19 |
| Strain Name | 379 | Sampling Date | from 2013-12 to 2015-01 |
| Sampling Country | Iran | Location | Iran |
| Submitted By | Ghavami,N., Ajaminejadfard,F., Sarshari,B., Meysami,P., Mokhtari Azad,T. and Rezaei,F. | Submitting Institution | Virology Department, School of Public Health, Tehran University of Medical Sciences, Keshavarz Blvd., Vali-e-Asr Square, Tehran 14155-6559, Iran |
| Original Host | Homo sapiens | Sample Type | Serum Samples |
| Standardized Host Name | Homo sapiens | Host Tax Rank | species |
| Standardized Host Class | Mammalia | Standardized Host Order | Primates |
| Environmental Origin | - N/A - | Reagent Origin | - N/A - |
| Number | Reagent/Consumables | Purpose | PMID/Url | Reference |
|---|---|---|---|---|
| 1 | Siemens Novagnost ELISA kits | Used for detecting anti-B19V IgM and IgG antibodies in serum samples. | 26538067 | View Related Publication |
| 2 | High Pure viral nucleic acid kit (Roche Diagnostics, Germany) | Used for extracting viral DNA from serum. | ||
| 3 | TaqMan Universal PCR Master Mix (Applied Biosystems, Carlsbad CA) | Used in Real-Time PCR for the quantification of B19V DNA. | ||
| 4 | B19V standard DNA (Primerdesign, UK) | Used as a standard in Real-Time PCR for quantifying B19V DNA. | ||
| 5 | Platinum1PCR SuperMix High Fidelity | Used for performing first-round PCR to obtain a DNA fragment for genotyping. | ||
| 6 | QIAquick gel extraction kit (Qiagen, Germany) | Used for gel purification of PCR products. | ||
| 7 | BigDye terminator cycle sequencing kit (Applied Biosystems) | Used for bidirectional sequencing of PCR products to determine B19V genotype. |